Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 839488..840052 Replicon chromosome
Accession NZ_CP027768
Organism Tetragenococcus halophilus strain LMG 26042

Toxin (Protein)


Gene name mazF Uniprot ID A0A2H6CPR8
Locus tag C7H83_RS04255 Protein ID WP_014123850.1
Coordinates 839717..840052 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3G5FHJ2
Locus tag C7H83_RS04250 Protein ID WP_041591760.1
Coordinates 839488..839724 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7H83_RS04230 835767..836318 + 552 WP_068710626.1 tyrosine-type recombinase/integrase -
C7H83_RS04235 836486..837904 - 1419 WP_123985489.1 ISLre2 family transposase -
C7H83_RS04240 838009..838518 - 510 Protein_797 UPF0236 family protein -
C7H83_RS04245 838878..838979 - 102 WP_069028690.1 putative holin-like toxin -
C7H83_RS04250 839488..839724 + 237 WP_041591760.1 hypothetical protein Antitoxin
C7H83_RS04255 839717..840052 + 336 WP_014123850.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C7H83_RS04260 840326..840640 + 315 WP_174706066.1 hypothetical protein -
C7H83_RS04265 840805..841170 - 366 WP_061840069.1 hotdog fold thioesterase -
C7H83_RS04270 841434..842252 + 819 WP_103892256.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
C7H83_RS04275 842345..843796 + 1452 WP_103892255.1 o-succinylbenzoate--CoA ligase -
C7H83_RS04280 844003..844725 + 723 WP_103892254.1 polyphosphate polymerase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12890.98 Da        Isoelectric Point: 8.9118

>T100293 WP_014123850.1 NZ_CP027768:839717-840052 [Tetragenococcus halophilus]
MTNHYTPEKGDIVWIDFDPSTGMEIQKRRPALVISRGNFNTATKFAVVCPITSTEIDLPTRYSLSENYKTKGQIVIHQMK
SLDFNKRKIDFIEKMNPVDLKKIEQIIEFII

Download         Length: 336 bp

>T100293 NZ_CP027768:839717-840052 [Tetragenococcus halophilus]
ATGACTAATCACTATACACCAGAAAAAGGAGACATAGTGTGGATCGACTTTGATCCTTCAACTGGAATGGAAATTCAAAA
GCGACGGCCAGCTCTGGTAATTTCGCGCGGAAATTTTAATACGGCGACAAAATTTGCGGTGGTATGTCCTATTACATCCA
CTGAAATAGATTTACCAACTAGATATAGTCTTTCAGAAAATTATAAAACAAAAGGACAAATAGTCATCCATCAAATGAAA
TCTTTAGATTTCAATAAACGCAAAATTGACTTTATTGAGAAAATGAACCCTGTGGACTTGAAGAAAATAGAACAGATCAT
TGAGTTCATTATTTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8506.60 Da        Isoelectric Point: 4.0338

>AT100293 WP_041591760.1 NZ_CP027768:839488-839724 [Tetragenococcus halophilus]
MLKTKTRAQGRSIVVTLPAEDGATVFPGKEYLVSYGNDGTIVLIPKIEDPFANVTEGAFYESDVWEDMPAVGKEVLDD

Download         Length: 237 bp

>AT100293 NZ_CP027768:839488-839724 [Tetragenococcus halophilus]
ATGCTAAAGACAAAAACAAGGGCACAAGGTAGATCTATCGTGGTTACTTTACCAGCTGAGGATGGGGCAACAGTTTTTCC
TGGGAAAGAATATCTTGTTTCATACGGAAATGATGGAACTATTGTTTTGATACCTAAAATTGAAGATCCGTTTGCTAATG
TTACAGAAGGAGCTTTTTACGAATCGGATGTATGGGAAGATATGCCAGCAGTTGGTAAAGAGGTGCTGGATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H6CPR8

Antitoxin

Source ID Structure
AlphaFold DB A0A3G5FHJ2

References