Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5074130..5074784 Replicon chromosome
Accession NZ_CP027766
Organism Escherichia coli strain 2013C-3342

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag C7M85_RS27380 Protein ID WP_096148702.1
Coordinates 5074377..5074784 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C7M85_RS27375 Protein ID WP_000354046.1
Coordinates 5074130..5074396 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7M85_RS27350 5069299..5070042 + 744 WP_000951964.1 SDR family oxidoreductase -
C7M85_RS27355 5070099..5071532 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
C7M85_RS27360 5071577..5071888 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C7M85_RS27365 5072052..5072711 + 660 WP_000250274.1 hemolysin III family protein -
C7M85_RS27370 5072907..5073887 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
C7M85_RS27375 5074130..5074396 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C7M85_RS27380 5074377..5074784 + 408 WP_096148702.1 protein YgfX Toxin
C7M85_RS27385 5074824..5075345 - 522 WP_001055874.1 flavodoxin FldB -
C7M85_RS27390 5075457..5076353 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C7M85_RS27395 5076378..5077088 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C7M85_RS27400 5077094..5078827 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15999.90 Da        Isoelectric Point: 11.5202

>T100284 WP_096148702.1 NZ_CP027766:5074377-5074784 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSVDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T100284 NZ_CP027766:5074377-5074784 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCGTGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT100284 WP_000354046.1 NZ_CP027766:5074130-5074396 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT100284 NZ_CP027766:5074130-5074396 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References