Detailed information of TA system TA100281

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 3610108..3610605
• Replicon: chromosome
• Accession: NZ_CP027766
• Organism: Escherichia coli strain 2013C-3342

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: C7M85_RS19745
• Protein ID: WP_001173343.1
• Coordinates: 3610108..3610281 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: C7M85_RS19750
• Protein ID: WP_071829713.1
• Coordinates: 3610309..3610605 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M85_RS19735	3606778..3608235	+	1458	WP_106883945.1	dipeptide/tripeptide permease DtpC	-
C7M85_RS19740	3608472..3609989	+	1518	WP_001295074.1	lysine--tRNA ligase	-
C7M85_RS19745	3610108..3610281	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
C7M85_RS19750	3610309..3610605	-	297	WP_071829713.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
C7M85_RS19760	3610832..3611104	-	273	WP_106883944.1	N-acetyltransferase	-
C7M85_RS19765	3611116..3611346	-	231	WP_000371700.1	(4Fe-4S)-binding protein	-
C7M85_RS19770	3611527..3613158	+	1632	WP_001216487.1	sensor histidine kinase	-
C7M85_RS19775	3613155..3613874	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	espG / nleE / nleB1 / nleB1 / espL2 / espF / escG / escF / cesD2 / espB / espD / espA / sepL / escD / eae / cesT / map / cesF / sepQ/escQ / escO / escN / escV / cesL / escI / escJ / sepD / escC / cesD / etgA / escU / escT / escS / escR / escL / escK / cesAB / escE	3545280..3610605	65325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T100281 WP_001173343.1 NZ_CP027766:c3610281-3610108 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T100281 NZ_CP027766:c3610281-3610108 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11453.95 Da
• Isoelectric Point: 4.1575

>AT100281 WP_071829713.1 NZ_CP027766:c3610605-3610309 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIDDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT100281 NZ_CP027766:c3610605-3610309 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGATGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41