Detailed information of TA system TA100280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3454530..3455125
• Replicon: chromosome
• Accession: NZ_CP027766
• Organism: Escherichia coli strain 2013C-3342

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: C7M85_RS18835
• Protein ID: WP_044805826.1
• Coordinates: 3454530..3454880 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: C7M85_RS18840
• Protein ID: WP_001223208.1
• Coordinates: 3454874..3455125 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M85_RS18815	3449976..3450998	-	1023	WP_001296689.1	ABC transporter permease	-
C7M85_RS18820	3451012..3452514	-	1503	WP_000205813.1	sugar ABC transporter ATP-binding protein	-
C7M85_RS18825	3452654..3453610	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
C7M85_RS18830	3453920..3454450	+	531	WP_044805825.1	inorganic diphosphatase	-
C7M85_RS18835	3454530..3454880	-	351	WP_044805826.1	endoribonuclease toxin ChpB	Toxin
C7M85_RS18840	3454874..3455125	-	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
C7M85_RS18845	3455337..3455678	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
C7M85_RS18850	3455681..3459460	-	3780	WP_106883961.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-115)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12479.39 Da
• Isoelectric Point: 5.6219

>T100280 WP_044805826.1 NZ_CP027766:c3454880-3454530 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMVDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T100280 NZ_CP027766:c3454880-3454530 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGGTGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT100280 WP_001223208.1 NZ_CP027766:c3455125-3454874 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT100280 NZ_CP027766:c3455125-3454874 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26