Detailed information of TA system TA100274

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2030610..2031442
• Replicon: chromosome
• Accession: NZ_CP027766
• Organism: Escherichia coli strain 2013C-3342

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1L4HFA4
• Locus tag: C7M85_RS11475
• Protein ID: WP_032163901.1
• Coordinates: 2031068..2031442 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C7M85_RS11470
• Protein ID: WP_032163902.1
• Coordinates: 2030610..2030978 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M85_RS11435	2027500..2027955	+	456	WP_000581504.1	hypothetical protein	-
C7M85_RS11440	2028034..2028267	+	234	WP_001119729.1	DUF905 family protein	-
C7M85_RS11450	2028367..2029185	+	819	WP_044805320.1	DUF945 domain-containing protein	-
C7M85_RS11455	2029276..2029761	+	486	WP_044805322.1	antirestriction protein	-
C7M85_RS11460	2029776..2030252	+	477	WP_159028785.1	RadC family protein	-
C7M85_RS11465	2030315..2030536	+	222	WP_000692323.1	DUF987 domain-containing protein	-
C7M85_RS11470	2030610..2030978	+	369	WP_032163902.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
C7M85_RS11475	2031068..2031442	+	375	WP_032163901.1	TA system toxin CbtA family protein	Toxin
C7M85_RS11480	2031439..2031633	+	195	WP_000988599.1	hypothetical protein	-
C7M85_RS30535	2031646..2031759	+	114	WP_001161660.1	DUF957 domain-containing protein	-
C7M85_RS11490	2032048..2032194	-	147	Protein_2108	transposase domain-containing protein	-
C7M85_RS11495	2032248..2032430	+	183	WP_001016348.1	hypothetical protein	-
C7M85_RS11500	2032531..2032860	-	330	WP_000450409.1	DUF496 family protein	-
C7M85_RS11505	2033032..2034090	-	1059	WP_044805323.1	FUSC family protein	-
C7M85_RS11510	2034288..2034761	-	474	WP_001105393.1	DNA gyrase inhibitor SbmC	-
C7M85_RS11515	2034880..2036046	-	1167	WP_001320295.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13919.89 Da
• Isoelectric Point: 7.7760

>T100274 WP_032163901.1 NZ_CP027766:2031068-2031442 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T100274 NZ_CP027766:2031068-2031442 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13501.35 Da
• Isoelectric Point: 6.6290

>AT100274 WP_032163902.1 NZ_CP027766:2030610-2030978 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT100274 NZ_CP027766:2030610-2030978 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1L4HFA4