Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1362832..1363303 Replicon chromosome
Accession NZ_CP027766
Organism Escherichia coli strain 2013C-3342

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C7M85_RS07395 Protein ID WP_001303511.1
Coordinates 1363025..1363303 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C7M85_RS07390 Protein ID WP_001302048.1
Coordinates 1362832..1363023 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7M85_RS07350 1358731..1359309 - 579 WP_001204666.1 sce7726 family protein -
C7M85_RS07355 1359615..1359926 - 312 WP_001002672.1 hypothetical protein -
C7M85_RS07360 1359919..1360173 - 255 WP_000004322.1 hypothetical protein -
C7M85_RS07365 1360170..1360592 - 423 WP_001151120.1 DUF977 family protein -
C7M85_RS07370 1360633..1361595 - 963 WP_000095671.1 helix-turn-helix domain-containing protein -
C7M85_RS07375 1361618..1362043 - 426 WP_000693943.1 Rha family transcriptional regulator -
C7M85_RS07380 1362040..1362342 - 303 WP_106884078.1 transcriptional regulator -
C7M85_RS07385 1362440..1362811 + 372 WP_001169687.1 hypothetical protein -
C7M85_RS07390 1362832..1363023 + 192 WP_001302048.1 hypothetical protein Antitoxin
C7M85_RS07395 1363025..1363303 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C7M85_RS07405 1363656..1363955 + 300 WP_001240334.1 hypothetical protein -
C7M85_RS07410 1364027..1364245 + 219 WP_001171930.1 hypothetical protein -
C7M85_RS07420 1364814..1365002 + 189 WP_000449192.1 cell division inhibition protein DicB -
C7M85_RS07425 1364999..1365190 + 192 WP_001090200.1 DUF1482 family protein -
C7M85_RS07430 1365283..1367754 + 2472 WP_106878764.1 exonuclease -
C7M85_RS07435 1367816..1368085 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1324322..1376341 52019


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T100264 WP_001303511.1 NZ_CP027766:1363025-1363303 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T100264 NZ_CP027766:1363025-1363303 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT100264 WP_001302048.1 NZ_CP027766:1362832-1363023 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT100264 NZ_CP027766:1362832-1363023 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References