Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1152113..1152751 Replicon chromosome
Accession NZ_CP027766
Organism Escherichia coli strain 2013C-3342

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C7M85_RS06265 Protein ID WP_000813794.1
Coordinates 1152575..1152751 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C7M85_RS06260 Protein ID WP_001270286.1
Coordinates 1152113..1152529 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7M85_RS06240 1147265..1148206 - 942 WP_106884109.1 ABC transporter permease -
C7M85_RS06245 1148207..1149220 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C7M85_RS06250 1149238..1150383 - 1146 WP_044805956.1 ABC transporter substrate-binding protein -
C7M85_RS06255 1150628..1152034 - 1407 WP_044805955.1 PLP-dependent aminotransferase family protein -
C7M85_RS06260 1152113..1152529 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C7M85_RS06265 1152575..1152751 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C7M85_RS06270 1152973..1153203 + 231 WP_000494244.1 YncJ family protein -
C7M85_RS06275 1153295..1155256 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C7M85_RS06280 1155329..1155865 - 537 WP_106884108.1 DNA-binding transcriptional regulator SutR -
C7M85_RS06285 1155918..1157132 + 1215 WP_001307190.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1157172..1158320 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T100263 WP_000813794.1 NZ_CP027766:c1152751-1152575 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T100263 NZ_CP027766:c1152751-1152575 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT100263 WP_001270286.1 NZ_CP027766:c1152529-1152113 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT100263 NZ_CP027766:c1152529-1152113 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References