Detailed information of TA system TA100261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 63549..64174
• Replicon: chromosome
• Accession: NZ_CP027766
• Organism: Escherichia coli strain 2013C-3342

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C7M85_RS00310
• Protein ID: WP_044805154.1
• Coordinates: 63776..64174 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C7M85_RS00305
• Protein ID: WP_000450524.1
• Coordinates: 63549..63776 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M85_RS00280	59352..59822	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C7M85_RS00285	59822..60394	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C7M85_RS00290	60540..61418	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C7M85_RS00295	61435..62469	+	1035	WP_106884216.1	outer membrane protein assembly factor BamC	-
C7M85_RS00300	62682..63395	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C7M85_RS00305	63549..63776	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C7M85_RS00310	63776..64174	+	399	WP_044805154.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C7M85_RS00315	64321..65184	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
C7M85_RS00320	65199..67214	+	2016	WP_044805153.1	tRNA cytosine(34) acetyltransferase TmcA	-
C7M85_RS00325	67288..67986	+	699	WP_000679812.1	esterase	-
C7M85_RS00330	68096..68296	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14924.28 Da
• Isoelectric Point: 8.5325

>T100261 WP_044805154.1 NZ_CP027766:63776-64174 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVREWFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T100261 NZ_CP027766:63776-64174 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGTGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT100261 WP_000450524.1 NZ_CP027766:63549-63776 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT100261 NZ_CP027766:63549-63776 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33