Detailed information of TA system TA100245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2951100..2951875
• Replicon: chromosome
• Accession: NZ_CP027760
• Organism: Pseudomonas sp. R4-34-07

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: C4J85_RS13450
• Protein ID: WP_124409867.1
• Coordinates: 2951100..2951600 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: C4J85_RS13455
• Protein ID: WP_124409868.1
• Coordinates: 2951597..2951875 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J85_RS13425	2946280..2947785	-	1506	WP_124409862.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
C4J85_RS13430	2947782..2948123	-	342	WP_124409863.1	hypothetical protein	-
C4J85_RS13435	2948125..2949480	-	1356	WP_124409864.1	integrating conjugative element protein	-
C4J85_RS13440	2949477..2950415	-	939	WP_124409865.1	TIGR03756 family integrating conjugative element protein	-
C4J85_RS13445	2950549..2950848	-	300	WP_124409866.1	hypothetical protein	-
C4J85_RS13450	2951100..2951600	-	501	WP_124409867.1	GNAT family N-acetyltransferase	Toxin
C4J85_RS13455	2951597..2951875	-	279	WP_124409868.1	DUF1778 domain-containing protein	Antitoxin
C4J85_RS13460	2951941..2952444	-	504	WP_124409869.1	DNA repair protein RadC	-
C4J85_RS13465	2953110..2955944	-	2835	WP_124409870.1	conjugative transfer ATPase	-
C4J85_RS13470	2955944..2956354	-	411	WP_124409871.1	TIGR03751 family conjugal transfer lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-91)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18232.15 Da
• Isoelectric Point: 9.1767

>T100245 WP_124409867.1 NZ_CP027760:c2951600-2951100 [Pseudomonas sp. R4-34-07]
MMLCAPQPLDASHVLESFDCGKPVLNEWLIRHARQAQSSGSARTFVVLDDRRLVGYFSLTVGQVDVLTAPERISRGMGRY
PIPVAILARMAVSGVDQGRGIGVGMLREAIARTLTVADQVGVRALLTHPLDEEAARFYRRFGFESSPASESQLLLLLKDA
RTLLVR

Nucleotide

• Length: 501 bp

>T100245 NZ_CP027760:c2951600-2951100 [Pseudomonas sp. R4-34-07]
CTGATGCTCTGTGCCCCGCAACCGTTGGATGCCAGCCATGTGCTGGAATCCTTCGACTGCGGTAAACCGGTGCTTAACGA
GTGGCTGATCCGACATGCCCGCCAGGCACAATCCAGCGGTTCGGCCCGAACCTTCGTGGTACTGGACGACCGGCGACTGG
TCGGGTATTTCAGTCTGACCGTTGGCCAAGTCGATGTCCTCACGGCCCCGGAACGGATCAGCCGTGGCATGGGCCGCTAT
CCAATCCCCGTGGCGATCCTGGCACGCATGGCGGTATCGGGTGTCGATCAGGGCCGCGGTATCGGTGTCGGGATGTTGCG
CGAGGCGATTGCGCGCACGTTGACGGTAGCCGATCAGGTCGGTGTGCGGGCCTTGCTGACCCATCCATTGGATGAGGAGG
CGGCACGCTTCTACCGGCGGTTCGGCTTCGAATCCTCCCCAGCCAGCGAATCGCAACTGCTGTTGCTGCTCAAAGACGCG
CGCACCCTACTCGTACGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10262.65 Da
• Isoelectric Point: 4.9802

>AT100245 WP_124409868.1 NZ_CP027760:c2951875-2951597 [Pseudomonas sp. R4-34-07]
MTISTPRSSRLGLRTTPQQDTVLRRAAKLANKSLTDFILDSACQVAEQTLLDQRLFLLDGEQAQAFVELLDRPVQDNPGL
QDLLSRPAPWSN

Nucleotide

• Length: 279 bp

>AT100245 NZ_CP027760:c2951875-2951597 [Pseudomonas sp. R4-34-07]
ATGACCATCAGCACGCCGCGCAGCTCCCGACTCGGCCTGCGCACTACCCCACAGCAAGACACCGTCCTGCGTCGGGCGGC
CAAATTGGCTAACAAATCGTTAACCGATTTCATCCTCGACAGTGCCTGTCAGGTCGCCGAGCAGACCCTGCTCGATCAGC
GGCTGTTCCTGCTCGACGGCGAACAAGCTCAGGCCTTTGTAGAGCTGCTCGACCGGCCCGTACAGGACAATCCTGGCCTA
CAGGACCTGCTGAGCCGTCCTGCACCCTGGAGCAACTGA