Detailed information of TA system TA100243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1800883..1801487
• Replicon: chromosome
• Accession: NZ_CP027760
• Organism: Pseudomonas sp. R4-34-07

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G8C3H2
• Locus tag: C4J85_RS08280
• Protein ID: WP_057722727.1
• Coordinates: 1800883..1801071 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J85_RS08285
• Protein ID: WP_124409409.1
• Coordinates: 1801083..1801487 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J85_RS08250	1796462..1796998	+	537	WP_124361949.1	DsbE family thiol:disulfide interchange protein	-
C4J85_RS08255	1796995..1797468	+	474	WP_124366413.1	cytochrome c-type biogenesis protein CcmH	-
C4J85_RS08260	1797461..1798645	+	1185	WP_124409407.1	c-type cytochrome biogenesis protein CcmI	-
C4J85_RS08265	1798661..1799056	+	396	WP_124370154.1	hypothetical protein	-
C4J85_RS08270	1799215..1799478	-	264	WP_124370155.1	DUF3077 domain-containing protein	-
C4J85_RS08275	1800138..1800704	+	567	WP_124409408.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
C4J85_RS08280	1800883..1801071	+	189	WP_057722727.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4J85_RS08285	1801083..1801487	+	405	WP_124409409.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J85_RS08290	1801484..1802680	-	1197	WP_124406711.1	MFS transporter	-
C4J85_RS08295	1802905..1803909	-	1005	WP_124409410.1	sulfate ABC transporter substrate-binding protein	-
C4J85_RS08300	1804005..1804829	-	825	WP_124409411.1	ion transporter	-
C4J85_RS08305	1804861..1805736	-	876	WP_124409412.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7204.45 Da
• Isoelectric Point: 11.2000

>T100243 WP_057722727.1 NZ_CP027760:1800883-1801071 [Pseudomonas sp. R4-34-07]
VQSRLLIKELLEAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T100243 NZ_CP027760:1800883-1801071 [Pseudomonas sp. R4-34-07]
GTGCAAAGCAGGCTATTGATCAAGGAATTGCTGGAAGCGGGTTGGGTGCTGGATCGCGTCACCGGCAGCCATCACATTTT
TACCCACCGCTATAACCCGTACACGATTCCGGTGCCTCATCCGAAGAAGGATCTTCCGTTGGGCACCGTCAGAAGCATCA
GGAAACGTGCCGGGCTGTTTCAGTTTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14733.72 Da
• Isoelectric Point: 4.6000

>AT100243 WP_124409409.1 NZ_CP027760:1801083-1801487 [Pseudomonas sp. R4-34-07]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGVIPMPTSVANHHTHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT100243 NZ_CP027760:1801083-1801487 [Pseudomonas sp. R4-34-07]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATTCCGGGTGCTGT
CACCGCCGGGGACAGTTTTGAAGAGGCGTACAACGCGGCCGTGGAGGTTGCACACATCATGCTGCAGGAGATCGCCGCCG
AGGGCGGAGTGATTCCGATGCCAACCTCAGTCGCCAATCATCACACCCATGAAGACTACGCCGGCATGGGCTGGGGCATG
TTGGAGCTGGACATCTCGCCCTATCTGGGCAAGACCGAGAAGGTCAACGTGACCTTGCCTGGGTATGTGATCCAACGCAT
CGACCGTTACGTGCGCGAGCATAAAGTCAAAAGCCGCTCGTCATTTCTGGCGGACGCGGCGTTGGAGAAGTTGGTGCGTT
CTTAA

Structures

Toxin

• AlphaFold DB: A0A3G8C3H2