Detailed information of TA system TA100236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1986537..1987141
• Replicon: chromosome
• Accession: NZ_CP027759
• Organism: Pseudomonas sp. R2-7-07

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G8C3H2
• Locus tag: C4J86_RS09125
• Protein ID: WP_057722727.1
• Coordinates: 1986537..1986725 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J86_RS09130
• Protein ID: WP_048729935.1
• Coordinates: 1986737..1987141 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J86_RS09095	1982114..1982650	+	537	WP_124361949.1	DsbE family thiol:disulfide interchange protein	-
C4J86_RS09100	1982647..1983120	+	474	WP_124366413.1	cytochrome c-type biogenesis protein CcmH	-
C4J86_RS09105	1983113..1984297	+	1185	WP_124370153.1	c-type cytochrome biogenesis protein CcmI	-
C4J86_RS09110	1984313..1984708	+	396	WP_124370154.1	hypothetical protein	-
C4J86_RS09115	1984867..1985130	-	264	WP_124370155.1	DUF3077 domain-containing protein	-
C4J86_RS09120	1985790..1986356	+	567	WP_124361954.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
C4J86_RS09125	1986537..1986725	+	189	WP_057722727.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4J86_RS09130	1986737..1987141	+	405	WP_048729935.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J86_RS09135	1987138..1988334	-	1197	WP_124370156.1	MFS transporter	-
C4J86_RS09140	1988574..1989578	-	1005	WP_124370157.1	sulfate ABC transporter substrate-binding protein	-
C4J86_RS09145	1989675..1990499	-	825	WP_124361959.1	ion transporter	-
C4J86_RS09150	1990531..1991406	-	876	WP_124370158.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7204.45 Da
• Isoelectric Point: 11.2000

>T100236 WP_057722727.1 NZ_CP027759:1986537-1986725 [Pseudomonas sp. R2-7-07]
VQSRLLIKELLEAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T100236 NZ_CP027759:1986537-1986725 [Pseudomonas sp. R2-7-07]
GTGCAAAGCAGGCTATTGATCAAGGAATTGCTGGAAGCGGGTTGGGTGCTGGATCGCGTCACCGGCAGCCATCACATTTT
TACCCACCGCTATAACCCGTACACGATCCCGGTGCCTCATCCGAAGAAGGATCTTCCCTTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGGCTGTTTCAGTTTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14703.69 Da
• Isoelectric Point: 4.6000

>AT100236 WP_048729935.1 NZ_CP027759:1986737-1987141 [Pseudomonas sp. R2-7-07]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGVIPMPTSVANHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT100236 NZ_CP027759:1986737-1987141 [Pseudomonas sp. R2-7-07]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATTCCGGGTGCTGT
CACCGCCGGGGACAGTTTTGAAGAGGCGTATAACGCGGCCGTGGAGGTTGCACACATCATGCTGCAGGAGATCGCCGCCG
AGGGCGGAGTGATTCCGATGCCAACCTCAGTCGCCAATCATCACGCCCATGAAGACTACGCCGGCATGGGCTGGGGCATG
TTGGAACTGGATATCTCGCCCTATCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCCGGCTATGTCATCCAGCGTAT
CGACCGTTACGTGCGCGAGCATAAAGTCAAAAGCCGCTCGTCATTTCTGGCGGACGCGGCGTTGGAGAAGTTGGTGCGGT
CTTAA

Structures

Toxin

• AlphaFold DB: A0A3G8C3H2