Detailed information of TA system TA100235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 1643478..1643991
• Replicon: chromosome
• Accession: NZ_CP027759
• Organism: Pseudomonas sp. R2-7-07

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4J86_RS07365
• Protein ID: WP_124361707.1
• Coordinates: 1643478..1643762 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4J86_RS07370
• Protein ID: WP_124361708.1
• Coordinates: 1643752..1643991 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J86_RS07340	1638711..1639859	+	1149	WP_124369936.1	L-threonine dehydrogenase	-
C4J86_RS07345	1639951..1640625	+	675	WP_124369937.1	NAD-dependent epimerase/dehydratase family protein	-
C4J86_RS07350	1640718..1641909	+	1192	Protein_1419	CoA transferase	-
C4J86_RS07355	1641996..1642484	-	489	WP_124369938.1	DMT family transporter	-
C4J86_RS07360	1642562..1643470	+	909	WP_177413157.1	LysR family transcriptional regulator	-
C4J86_RS07365	1643478..1643762	-	285	WP_124361707.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J86_RS07370	1643752..1643991	-	240	WP_124361708.1	ribbon-helix-helix protein, CopG family	Antitoxin
C4J86_RS07375	1644092..1644502	+	411	WP_124369940.1	fosfomycin resistance glutathione transferase	-
C4J86_RS07380	1644672..1645199	+	528	WP_057723086.1	DUF4142 domain-containing protein	-
C4J86_RS07385	1645270..1645695	-	426	WP_124361712.1	low affinity iron permease family protein	-
C4J86_RS07390	1645872..1646654	+	783	WP_124369941.1	GNAT family N-acetyltransferase	-
C4J86_RS07395	1646655..1647155	-	501	WP_124369942.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
C4J86_RS07405	1647570..1648454	-	885	WP_124369943.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11014.73 Da
• Isoelectric Point: 10.1506

>T100235 WP_124361707.1 NZ_CP027759:c1643762-1643478 [Pseudomonas sp. R2-7-07]
MQVEWLRTALRNLDDEAAFIAEENPKAAYEFVQAILAGLEHIAQFPALGKTGRVPGTREWVVPRWPYIVPYRIREGRLQV
MRIFHTRRQPPTSW

Nucleotide

• Length: 285 bp

>T100235 NZ_CP027759:c1643762-1643478 [Pseudomonas sp. R2-7-07]
ATGCAGGTTGAGTGGCTGAGGACAGCCCTCAGAAACCTGGATGATGAAGCGGCCTTTATTGCTGAAGAAAACCCTAAAGC
CGCCTACGAGTTCGTCCAGGCGATTTTGGCCGGGCTTGAACACATTGCGCAATTTCCCGCGTTGGGAAAAACCGGGCGCG
TCCCAGGGACGCGTGAATGGGTGGTGCCCCGGTGGCCCTACATTGTCCCTTACCGTATTCGTGAAGGGCGACTACAGGTG
ATGCGAATTTTTCATACCCGCCGCCAACCACCTACCTCCTGGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8597.72 Da
• Isoelectric Point: 4.7571

>AT100235 WP_124361708.1 NZ_CP027759:c1643991-1643752 [Pseudomonas sp. R2-7-07]
MSVMSLRIPDDVADTLASLAKATGRSKSFLAVDALREYLTREAWQIEEIQKALKEADAGDFATAAEVKAVADKWRNNAG

Nucleotide

• Length: 240 bp

>AT100235 NZ_CP027759:c1643991-1643752 [Pseudomonas sp. R2-7-07]
ATGTCTGTTATGTCCCTGCGGATACCGGACGATGTCGCCGATACGCTTGCCAGTCTGGCCAAAGCGACCGGTCGCAGCAA
ATCGTTCCTCGCTGTTGACGCGCTACGTGAATACTTGACGCGTGAAGCATGGCAAATCGAAGAGATTCAAAAGGCGCTGA
AAGAAGCTGATGCGGGGGATTTCGCGACGGCTGCCGAGGTAAAAGCCGTCGCTGACAAATGGCGCAACAATGCAGGTTGA