Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/upstrm_HI1419-dnstrm_HI1420
Location 1677725..1678311 Replicon chromosome
Accession NZ_CP027758
Organism Pseudomonas synxantha strain R2-54-08W

Toxin (Protein)


Gene name relE Uniprot ID A0A6I6W8S7
Locus tag C4J99_RS07530 Protein ID WP_056788882.1
Coordinates 1678003..1678311 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0Q8YRR5
Locus tag C4J99_RS07525 Protein ID WP_056788879.1
Coordinates 1677725..1678006 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4J99_RS07490 1673770..1674264 + 495 WP_206426338.1 hypothetical protein -
C4J99_RS07495 1674264..1674506 + 243 WP_124399748.1 hypothetical protein -
C4J99_RS07500 1674523..1675215 + 693 WP_073454430.1 SOS response-associated peptidase -
C4J99_RS07505 1675253..1676014 + 762 WP_073454428.1 hypothetical protein -
C4J99_RS07510 1676111..1676431 + 321 WP_073454425.1 hypothetical protein -
C4J99_RS07515 1676550..1677362 + 813 WP_073454423.1 hypothetical protein -
C4J99_RS07520 1677359..1677652 + 294 WP_124399749.1 hypothetical protein -
C4J99_RS07525 1677725..1678006 - 282 WP_056788879.1 putative addiction module antidote protein Antitoxin
C4J99_RS07530 1678003..1678311 - 309 WP_056788882.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C4J99_RS07535 1678369..1679127 - 759 WP_073454421.1 hypothetical protein -
C4J99_RS07540 1679127..1679648 - 522 WP_073454419.1 hypothetical protein -
C4J99_RS07545 1679659..1680093 - 435 WP_065948956.1 hypothetical protein -
C4J99_RS07550 1680162..1681232 - 1071 WP_124399750.1 hypothetical protein -
C4J99_RS07555 1681234..1681845 - 612 WP_073454413.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1656353..1750579 94226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-89)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11478.19 Da        Isoelectric Point: 7.8182

>T100228 WP_056788882.1 NZ_CP027758:c1678311-1678003 [Pseudomonas synxantha]
MNTIITTHEFDNWLKGLKDVQARLRIVSRILSAEAGNFGDCEPVGEGISEMRIFVGPGYRVYYTRRGEVVYLLLCGGNKS
SQKRDIKLAKSILDEIEQGDVK

Download         Length: 309 bp

>T100228 NZ_CP027758:c1678311-1678003 [Pseudomonas synxantha]
ATGAACACAATCATCACTACCCATGAATTTGATAACTGGCTGAAAGGTCTGAAAGACGTACAGGCACGTCTACGGATCGT
TTCTCGGATCTTGAGTGCAGAGGCGGGTAATTTTGGTGACTGTGAGCCGGTGGGCGAAGGCATCAGCGAAATGCGGATCT
TCGTCGGGCCGGGATACCGTGTGTACTACACGCGCCGTGGCGAGGTCGTTTACCTGCTACTGTGTGGCGGCAATAAGTCA
TCACAGAAACGAGACATCAAGCTTGCCAAATCCATCCTCGATGAGATCGAGCAAGGAGACGTAAAATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10287.83 Da        Isoelectric Point: 7.0104

>AT100228 WP_056788879.1 NZ_CP027758:c1678006-1677725 [Pseudomonas synxantha]
MRELKAFDASQYLTNEDEIAQFLTISLEDPNPDVFLRALQEVAKARGMSQLAKDAGINRESLYKALAPGAKPRYDTVLKL
VNALGVKLTAQHV

Download         Length: 282 bp

>AT100228 NZ_CP027758:c1678006-1677725 [Pseudomonas synxantha]
ATGAGAGAACTCAAAGCATTTGATGCATCCCAGTACCTCACCAACGAGGACGAGATTGCTCAATTCCTGACCATTTCTTT
GGAAGACCCAAACCCTGACGTGTTCCTTCGCGCTCTGCAGGAAGTCGCCAAGGCTCGTGGCATGAGCCAGCTTGCTAAGG
ATGCTGGTATCAATCGTGAGAGCCTGTACAAAGCGTTGGCACCAGGTGCGAAGCCCCGGTATGACACAGTGTTGAAACTG
GTGAATGCATTAGGCGTGAAGCTAACGGCCCAGCACGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I6W8S7

Antitoxin

Source ID Structure
AlphaFold DB A0A0Q8YRR5

References