Detailed information of TA system TA100227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1614095..1614710
• Replicon: chromosome
• Accession: NZ_CP027758
• Organism: Pseudomonas synxantha strain R2-54-08W

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4J99_RS07205
• Protein ID: WP_082636614.1
• Coordinates: 1614528..1614710 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0R2Z981
• Locus tag: C4J99_RS07200
• Protein ID: WP_057022010.1
• Coordinates: 1614095..1614505 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J99_RS07165	1609905..1610321	+	417	WP_124399706.1	fosfomycin resistance glutathione transferase	-
C4J99_RS07170	1610369..1611010	+	642	WP_124376839.1	LysE family translocator	-
C4J99_RS07175	1611131..1611658	+	528	WP_005785958.1	DUF4142 domain-containing protein	-
C4J99_RS07180	1611714..1612139	-	426	WP_124399707.1	low affinity iron permease family protein	-
C4J99_RS07185	1612318..1613100	+	783	WP_124399708.1	GNAT family N-acetyltransferase	-
C4J99_RS07190	1613101..1613601	-	501	WP_124399709.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
C4J99_RS07195	1613701..1614012	-	312	WP_124399710.1	hypothetical protein	-
C4J99_RS07200	1614095..1614505	-	411	WP_057022010.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J99_RS07205	1614528..1614710	-	183	WP_082636614.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4J99_RS07210	1614877..1615770	-	894	WP_124399711.1	LysR family transcriptional regulator	-
C4J99_RS07215	1615866..1617029	+	1164	WP_124399712.1	MFS transporter	-
C4J99_RS07225	1617350..1618234	-	885	WP_124399713.1	alpha/beta hydrolase	-
C4J99_RS07230	1618540..1619232	-	693	WP_124399714.1	pseudouridine synthase	-
C4J99_RS07235	1619222..1619434	-	213	WP_124399715.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6832.02 Da
• Isoelectric Point: 10.8600

>T100227 WP_082636614.1 NZ_CP027758:c1614710-1614528 [Pseudomonas synxantha]
VDSRYLIGHIVADGWYLVRIRGSHHHFKHPTKPGLVTIPHPKKDLLDKTAKSILRQALLS

Nucleotide

• Length: 183 bp

>T100227 NZ_CP027758:c1614710-1614528 [Pseudomonas synxantha]
GTGGACAGTCGATATTTGATCGGTCATATCGTTGCAGACGGTTGGTATCTTGTACGCATCAGGGGCAGTCATCACCATTT
CAAGCACCCGACGAAGCCAGGGTTGGTAACGATCCCTCATCCAAAAAAGGACCTGCTGGATAAGACCGCCAAGAGCATCT
TGAGACAGGCTCTGTTGAGTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14782.73 Da
• Isoelectric Point: 4.4720

>AT100227 WP_057022010.1 NZ_CP027758:c1614505-1614095 [Pseudomonas synxantha]
MLYPIAISTGDKDHAWGVEVPDIPGCYSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPSAQKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLNHPEEKSRSGFLASAALKVLQQDR

Nucleotide

• Length: 411 bp

>AT100227 NZ_CP027758:c1614505-1614095 [Pseudomonas synxantha]
ATGCTTTACCCCATCGCAATTTCAACCGGCGACAAGGACCACGCGTGGGGCGTGGAGGTCCCGGATATTCCAGGCTGCTA
CTCCGCCGGCGATGACCTGGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATCCTGGCAGAGG
ATGGCGCGCCGATCCCCAGCGCCCAGAAAGTCACCCTGCATGCCGCCAACCCACAGTACGCTGGCTGCACGTGGGCAGTG
GTGGACATCGATGTCACCAAGTACCTGGGCAAGGCGCAAAAACTCAACATCACGCTGCCGGGTTACCTGTTGAATCGCAT
CGATGAATATGTGTTGAATCATCCGGAAGAAAAAAGCCGCTCGGGGTTCCTGGCGTCGGCAGCGCTCAAGGTATTGCAGC
AGGACCGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0R2Z981