Detailed information of TA system TA100225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4783229..4783793
• Replicon: chromosome
• Accession: NZ_CP027757
• Organism: Pseudomonas synxantha strain R2-4-08W

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K00_RS21770
• Protein ID: WP_104505336.1
• Coordinates: 4783229..4783510 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4K00_RS21775
• Protein ID: WP_057013613.1
• Coordinates: 4783506..4783793 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K00_RS21750	4780031..4780300	+	270	WP_046069194.1	DUF2790 domain-containing protein	-
C4K00_RS21755	4780874..4782124	+	1251	WP_124412926.1	ribonucleotide-diphosphate reductase subunit beta	-
C4K00_RS21760	4782199..4782360	+	162	Protein_4203	HNH endonuclease	-
C4K00_RS21765	4782433..4783080	+	648	WP_124401646.1	HAD-IB family hydrolase	-
C4K00_RS21770	4783229..4783510	-	282	WP_104505336.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K00_RS21775	4783506..4783793	-	288	WP_057013613.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4K00_RS21780	4784054..4785013	-	960	WP_124401647.1	LysR family transcriptional regulator	-
C4K00_RS21785	4785207..4786634	+	1428	WP_124412927.1	argininosuccinate lyase	-
C4K00_RS21790	4786742..4787620	+	879	WP_124401649.1	amino acid ABC transporter permease	-
C4K00_RS21795	4787645..4788409	+	765	WP_124401650.1	amino acid ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-74)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10944.76 Da
• Isoelectric Point: 10.3141

>T100225 WP_104505336.1 NZ_CP027757:c4783510-4783229 [Pseudomonas synxantha]
IEFSIEWDPKALKELKKLDGTIRHQFLRKLQERQDHPRVPADALYGMKDCYKIKLRGAGFRLVYRVEDDRIVILVLAVGK
RERSTVYDSAKSR

Nucleotide

• Length: 282 bp

>T100225 NZ_CP027757:c4783510-4783229 [Pseudomonas synxantha]
ATTGAGTTTAGTATTGAGTGGGATCCGAAGGCGTTAAAAGAGCTCAAGAAACTGGACGGTACAATTCGTCATCAGTTTTT
GCGCAAACTTCAGGAGCGCCAAGATCACCCGCGGGTTCCAGCGGACGCGCTTTATGGAATGAAGGATTGCTACAAGATCA
AATTACGTGGTGCCGGCTTTCGGCTGGTCTACCGTGTTGAGGACGATCGAATAGTCATCCTCGTATTAGCGGTGGGTAAG
CGGGAACGTAGCACTGTCTACGATTCGGCCAAGAGCCGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10272.81 Da
• Isoelectric Point: 4.3557

>AT100225 WP_057013613.1 NZ_CP027757:c4783793-4783506 [Pseudomonas synxantha]
MQLVLADVAVSVSELKKNPSSVMNGANGSAVAVLNHNRVMGYMVPASVYEAMVERLDELELVQLVKERLDAKEVPIRVSL
DELIAEAEADIANGH

Nucleotide

• Length: 288 bp

>AT100225 NZ_CP027757:c4783793-4783506 [Pseudomonas synxantha]
ATGCAACTTGTTTTGGCTGACGTGGCCGTCAGCGTATCCGAACTCAAAAAGAACCCTTCATCCGTCATGAACGGAGCCAA
CGGCTCGGCGGTGGCTGTCCTCAACCATAATCGCGTGATGGGTTATATGGTCCCGGCTAGCGTATACGAGGCGATGGTTG
AGCGTCTTGATGAGCTGGAACTGGTGCAATTGGTCAAGGAGAGGTTGGACGCCAAGGAGGTACCGATTCGGGTTTCCCTT
GATGAGCTGATTGCTGAGGCAGAGGCGGATATCGCCAATGGGCATTGA