Detailed information of TA system TA100224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 4053253..4054470
• Replicon: chromosome
• Accession: NZ_CP027757
• Organism: Pseudomonas synxantha strain R2-4-08W

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C4K00_RS18205
• Protein ID: WP_124401235.1
• Coordinates: 4053898..4054470 (+)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C4K00_RS18200
• Protein ID: WP_124412665.1
• Coordinates: 4053253..4053810 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K00_RS18185	4048828..4049568	+	741	WP_056846683.1	molecular chaperone	-
C4K00_RS18190	4049654..4052164	+	2511	WP_124412664.1	fimbrial biogenesis outer membrane usher protein	-
C4K00_RS18195	4052224..4053060	+	837	WP_124401233.1	acid phosphatase	-
C4K00_RS18200	4053253..4053810	+	558	WP_124412665.1	helix-turn-helix domain-containing protein	Antitoxin
C4K00_RS18205	4053898..4054470	+	573	WP_124401235.1	HD domain-containing protein	Toxin
C4K00_RS18210	4054480..4054902	-	423	WP_046068825.1	RidA family protein	-
C4K00_RS18215	4054914..4056497	-	1584	WP_124401236.1	FAD-dependent oxidoreductase	-
C4K00_RS18220	4056510..4057433	-	924	WP_057024966.1	ABC transporter substrate-binding protein	-
C4K00_RS18225	4057452..4058225	-	774	WP_057013589.1	amino acid ABC transporter ATP-binding protein	-
C4K00_RS18230	4058218..4059189	-	972	WP_124412666.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (9-184)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-46)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21243.34 Da
• Isoelectric Point: 4.9633

>T100224 WP_124401235.1 NZ_CP027757:4053898-4054470 [Pseudomonas synxantha]
MDLEKIKGRLDFLREAEKLKDVLRSAHTASGRTESTAEHSWRLCLMAMVFADQLPGLDLLKILKMCVIHDLGEAIHGDIP
AVSQAAFPNKGEQERLDLLVLAQSLDSPLKAEILTLWDDYENALSPEAKAVKAMDKLETLLQHTQGLNPPDFDYAFNLAY
GKKHTDADPLFEALRALIDRATEQRLHAAE

Nucleotide

• Length: 573 bp

>T100224 NZ_CP027757:4053898-4054470 [Pseudomonas synxantha]
ATGGACCTGGAAAAAATCAAAGGCCGTCTCGATTTCCTGCGCGAAGCGGAAAAACTCAAGGATGTGCTCAGAAGTGCCCA
CACGGCTTCGGGTCGCACTGAAAGCACGGCCGAACACAGTTGGCGCCTGTGTCTGATGGCGATGGTGTTCGCCGATCAAC
TGCCCGGCCTGGACCTGCTGAAAATCCTCAAGATGTGCGTTATCCACGATTTGGGCGAAGCCATCCACGGCGATATCCCG
GCGGTCAGCCAGGCAGCCTTTCCCAACAAGGGCGAACAGGAGCGGCTCGACCTGCTGGTGTTGGCGCAGTCCCTGGACAG
CCCCCTGAAAGCCGAGATCCTGACGTTATGGGATGATTACGAGAACGCGCTTTCGCCGGAAGCAAAAGCGGTCAAGGCCA
TGGACAAACTGGAGACCCTGCTGCAGCACACCCAGGGGCTGAACCCTCCCGATTTCGACTACGCCTTCAACCTGGCCTAC
GGCAAAAAACACACGGATGCCGACCCCTTGTTCGAAGCATTGCGGGCATTGATCGATCGAGCGACAGAGCAAAGGCTTCA
CGCCGCCGAGTAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20563.57 Da
• Isoelectric Point: 8.3363

>AT100224 WP_124412665.1 NZ_CP027757:4053253-4053810 [Pseudomonas synxantha]
MPHPLLKPALPGIALRRWRLLHRVKQSHAAQLFNVTQSTISRWESGIQAMDPAAHQQLEQLLAARLDAAADQALARLVSE
SSQAMHLVCDLTHRLLACSPSRARQFSSPLTDLMGQSLWRFATQEIQLIEASLEHTGWRETQAPPALEFTTGSNDSSLVP
IRPSLCRWTRMTLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT100224 NZ_CP027757:4053253-4053810 [Pseudomonas synxantha]
ATGCCTCACCCCTTGCTCAAGCCTGCGCTGCCCGGCATTGCGCTGCGGCGATGGCGTTTGCTGCACCGCGTCAAGCAAAG
CCATGCGGCGCAGTTATTCAACGTCACTCAATCGACTATTTCACGCTGGGAAAGTGGCATACAAGCGATGGATCCGGCTG
CGCACCAACAGCTTGAGCAGCTGTTGGCAGCCCGGCTTGATGCAGCCGCCGACCAGGCATTGGCCCGGTTGGTCAGTGAG
AGTTCGCAAGCGATGCACCTGGTGTGCGACCTGACGCACCGCCTGCTCGCATGCTCGCCTTCCCGCGCACGCCAGTTCAG
CAGCCCGCTCACCGACCTTATGGGCCAGTCGCTGTGGCGCTTCGCAACCCAGGAAATCCAGCTGATCGAAGCCAGCCTGG
AGCACACCGGCTGGCGCGAAACCCAGGCACCCCCAGCCCTTGAGTTCACCACTGGAAGCAACGATTCCAGCCTCGTGCCG
ATACGCCCAAGCCTGTGTCGGTGGACGCGCATGACCCTGAGCGATGGCAGCGCCGCGCGCCTGGTCGAGACGCTCTGA