Detailed information of TA system TA100223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2587798..2588411
• Replicon: chromosome
• Accession: NZ_CP027757
• Organism: Pseudomonas synxantha strain R2-4-08W

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K00_RS11670
• Protein ID: WP_046072224.1
• Coordinates: 2588160..2588411 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K00_RS11665
• Protein ID: WP_046072223.1
• Coordinates: 2587798..2588163 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K00_RS11640	2583011..2583820	+	810	WP_124412087.1	ammonia-dependent NAD(+) synthetase	-
C4K00_RS11645	2583971..2584543	+	573	WP_124412088.1	DUF2239 family protein	-
C4K00_RS11650	2584566..2585921	+	1356	WP_124412089.1	MATE family efflux transporter	-
C4K00_RS11655	2585905..2586492	-	588	WP_124412090.1	TetR/AcrR family transcriptional regulator	-
C4K00_RS11660	2586625..2587797	+	1173	WP_124400367.1	MFS transporter	-
C4K00_RS11665	2587798..2588163	-	366	WP_046072223.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K00_RS11670	2588160..2588411	-	252	WP_046072224.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9546.13 Da
• Isoelectric Point: 10.0941

>T100223 WP_046072224.1 NZ_CP027757:c2588411-2588160 [Pseudomonas synxantha]
VSKNEKLLAKLLNEHMAFTWPELVTLLRRLGYTQIEGAGSRVKFDIGDPSAMITLHRPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100223 NZ_CP027757:c2588411-2588160 [Pseudomonas synxantha]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTTACCTGGCCCGAGCTCGTCACACTGTT
ACGACGGCTCGGATACACACAGATTGAAGGGGCCGGAAGCCGGGTCAAATTCGACATCGGCGACCCCAGTGCAATGATCA
CGTTGCACAGGCCTCACCCCGGCAACGAACTGAAGCACTACATTCGTCGCCAGATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13211.06 Da
• Isoelectric Point: 6.6273

>AT100223 WP_046072223.1 NZ_CP027757:c2588163-2587798 [Pseudomonas synxantha]
VNNQLKHNGYIGSIEASLEDNCLFGKVLFIKALVSYEGKTVAELEAAFREAVDDYLATCQALGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALSEFLQHHYPEPCPAIG

Nucleotide

• Length: 366 bp

>AT100223 NZ_CP027757:c2588163-2587798 [Pseudomonas synxantha]
GTGAACAACCAACTGAAACACAATGGCTATATCGGCTCTATCGAAGCCAGCCTTGAAGACAACTGCCTGTTTGGCAAGGT
CCTGTTCATCAAGGCATTAGTGAGCTACGAAGGTAAAACCGTCGCAGAACTGGAAGCCGCCTTCCGTGAAGCGGTGGACG
ATTACCTCGCGACCTGCCAGGCCCTCGGGCAAACGCCGGAGAAGCCTTGCAAAGGTTCATTCAACGTGCGCGTCGGGCAT
GACCTGCACTTGGCAGCAGCCCTGGCAGCAACCCGTAAGAAAGTGACGTTGAATGATCTGACGCGCCAAGCGTTGAGCGA
GTTTCTACAACATCACTATCCAGAGCCATGCCCGGCGATAGGCTGA