Detailed information of TA system TA100219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 5318146..5318732
• Replicon: chromosome
• Accession: NZ_CP027756
• Organism: Pseudomonas synxantha strain R6-28-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K01_RS24670
• Protein ID: WP_124356288.1
• Coordinates: 5318146..5318454 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0Q8YRR5
• Locus tag: C4K01_RS24675
• Protein ID: WP_056788879.1
• Coordinates: 5318451..5318732 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K01_RS24640	5313978..5314532	+	555	WP_115487771.1	hypothetical protein	-
C4K01_RS24645	5314603..5315223	+	621	WP_124356285.1	HEPN domain-containing protein	-
C4K01_RS24650	5315225..5316295	+	1071	WP_124356286.1	hypothetical protein	-
C4K01_RS24655	5316364..5316798	+	435	WP_065948956.1	hypothetical protein	-
C4K01_RS24660	5316809..5317330	+	522	WP_108538254.1	hypothetical protein	-
C4K01_RS24665	5317330..5318088	+	759	WP_124356287.1	hypothetical protein	-
C4K01_RS24670	5318146..5318454	+	309	WP_124356288.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K01_RS24675	5318451..5318732	+	282	WP_056788879.1	putative addiction module antidote protein	Antitoxin
C4K01_RS24680	5318815..5319600	-	786	WP_124356289.1	hypothetical protein	-
C4K01_RS24685	5319657..5319977	-	321	WP_124356290.1	hypothetical protein	-
C4K01_RS24690	5320054..5320785	-	732	WP_124356291.1	hypothetical protein	-
C4K01_RS24695	5321098..5321790	-	693	WP_124356292.1	SOS response-associated peptidase family protein	-
C4K01_RS24700	5321905..5323053	-	1149	WP_124356293.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5265264..5355961	90697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-89)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11504.22 Da
• Isoelectric Point: 7.8182

>T100219 WP_124356288.1 NZ_CP027756:5318146-5318454 [Pseudomonas synxantha]
MNTIITTHEFDNWLKGLKDVQPRLRIVSRILSAEAGNFGDCEPVGEGISEMRIFVGPGYRVYYTRRGEVVYLLLCGGNKS
SQKRDIKLAKSILDEIEQGDVK

Nucleotide

• Length: 309 bp

>T100219 NZ_CP027756:5318146-5318454 [Pseudomonas synxantha]
ATGAACACAATCATCACTACCCATGAATTTGATAACTGGCTGAAAGGTCTGAAAGACGTACAGCCACGTCTACGGATCGT
TTCTCGGATCTTGAGTGCAGAGGCGGGTAATTTTGGTGACTGTGAGCCGGTGGGCGAAGGCATCAGCGAAATGCGGATCT
TCGTCGGGCCGGGATACCGTGTGTACTACACGCGCCGTGGCGAGGTCGTTTACCTGCTACTGTGTGGCGGCAATAAGTCA
TCACAGAAACGAGACATCAAGCTTGCCAAATCCATCCTCGATGAGATCGAGCAAGGAGACGTAAAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10287.83 Da
• Isoelectric Point: 7.0104

>AT100219 WP_056788879.1 NZ_CP027756:5318451-5318732 [Pseudomonas synxantha]
MRELKAFDASQYLTNEDEIAQFLTISLEDPNPDVFLRALQEVAKARGMSQLAKDAGINRESLYKALAPGAKPRYDTVLKL
VNALGVKLTAQHV

Nucleotide

• Length: 282 bp

>AT100219 NZ_CP027756:5318451-5318732 [Pseudomonas synxantha]
ATGAGAGAACTCAAAGCATTTGATGCATCCCAGTACCTCACCAACGAGGACGAGATTGCTCAATTCCTGACCATTTCTTT
GGAAGACCCAAACCCTGACGTGTTCCTTCGCGCTCTGCAGGAAGTCGCCAAGGCTCGTGGCATGAGCCAGCTTGCCAAGG
ATGCTGGTATCAATCGTGAGAGCCTGTACAAAGCATTGGCACCGGGCGCAAAGCCCCGTTATGACACAGTGTTGAAGCTT
GTGAATGCGCTAGGTGTGAAGCTGACAGCCCAGCACGTTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0Q8YRR5