Detailed information of TA system TA100215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2688318..2688931
• Replicon: chromosome
• Accession: NZ_CP027756
• Organism: Pseudomonas synxantha strain R6-28-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K01_RS12490
• Protein ID: WP_046072224.1
• Coordinates: 2688680..2688931 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K01_RS12485
• Protein ID: WP_057022619.1
• Coordinates: 2688318..2688683 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K01_RS12460	2683532..2684341	+	810	WP_056846805.1	ammonia-dependent NAD(+) synthetase	-
C4K01_RS12465	2684492..2685064	+	573	WP_057022623.1	DUF2239 family protein	-
C4K01_RS12470	2685087..2686442	+	1356	WP_057022622.1	MATE family efflux transporter	-
C4K01_RS12475	2686426..2687013	-	588	WP_057022621.1	TetR/AcrR family transcriptional regulator	-
C4K01_RS12480	2687145..2688317	+	1173	WP_057022620.1	MFS transporter	-
C4K01_RS12485	2688318..2688683	-	366	WP_057022619.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K01_RS12490	2688680..2688931	-	252	WP_046072224.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9546.13 Da
• Isoelectric Point: 10.0941

>T100215 WP_046072224.1 NZ_CP027756:c2688931-2688680 [Pseudomonas synxantha]
VSKNEKLLAKLLNEHMAFTWPELVTLLRRLGYTQIEGAGSRVKFDIGDPSAMITLHRPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100215 NZ_CP027756:c2688931-2688680 [Pseudomonas synxantha]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTTACCTGGCCCGAGCTCGTCACACTGTT
ACGACGGCTCGGATACACACAGATTGAAGGGGCCGGAAGCCGGGTCAAATTCGACATCGGCGACCCCAGTGCAATGATCA
CGTTGCACAGGCCTCACCCCGGCAACGAACTGAAGCACTACATTCGTCGCCAGATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13220.07 Da
• Isoelectric Point: 6.7403

>AT100215 WP_057022619.1 NZ_CP027756:c2688683-2688318 [Pseudomonas synxantha]
VNNQLKHNGYIGSIEASLEDNCLFGKVLFIKALVSYEGKTVAELEAAFREAVDDYLATCHALGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALSEFLQHHYPEPCPAIG

Nucleotide

• Length: 366 bp

>AT100215 NZ_CP027756:c2688683-2688318 [Pseudomonas synxantha]
GTGAACAACCAACTGAAACACAATGGCTATATCGGCTCTATCGAAGCCAGCCTTGAAGACAACTGCCTGTTTGGCAAGGT
CCTGTTCATCAAGGCATTAGTGAGCTACGAAGGTAAAACCGTCGCAGAACTGGAAGCCGCCTTCCGTGAAGCGGTGGACG
ATTACCTCGCGACCTGCCATGCCCTCGGGCAAACGCCGGAGAAACCTTGCAAAGGTTCATTCAACGTGCGCGTCGGGCAT
GACCTGCACTTGGCAGCAGCCCTGGCAGCAACCCGTAAGAAAGTGACGTTGAATGATCTGACGCGCCAAGCGTTGAGCGA
GTTTCTACAACATCACTATCCAGAGCCATGCCCGGCGATAGGCTGA