Detailed information of TA system TA100212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1790363..1790885
• Replicon: chromosome
• Accession: NZ_CP027756
• Organism: Pseudomonas synxantha strain R6-28-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K01_RS08290
• Protein ID: WP_057022032.1
• Coordinates: 1790363..1790656 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0W0N4Z1
• Locus tag: C4K01_RS08295
• Protein ID: WP_014717442.1
• Coordinates: 1790646..1790885 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K01_RS08260	1785757..1786317	-	561	WP_003189463.1	glycine cleavage system protein R	-
C4K01_RS08265	1786579..1787457	+	879	WP_046071422.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C4K01_RS08270	1787475..1788590	+	1116	WP_057022030.1	outer membrane protein assembly factor BamC	-
C4K01_RS08275	1788595..1789353	+	759	WP_056847351.1	MBL fold metallo-hydrolase	-
C4K01_RS08280	1789381..1790094	+	714	WP_057022031.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C4K01_RS08290	1790363..1790656	-	294	WP_057022032.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K01_RS08295	1790646..1790885	-	240	WP_014717442.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C4K01_RS08305	1791539..1791793	+	255	WP_057022033.1	hypothetical protein	-
C4K01_RS08310	1791868..1793094	-	1227	WP_057022034.1	MFS transporter	-
C4K01_RS08315	1793334..1793621	-	288	WP_057022035.1	helix-turn-helix domain-containing protein	-
C4K01_RS08320	1793624..1793938	-	315	WP_124356083.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4K01_RS08325	1794044..1794985	-	942	WP_057022037.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	mucA / mucD	1780183..1803154	22971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11585.38 Da
• Isoelectric Point: 10.0977

>T100212 WP_057022032.1 NZ_CP027756:c1790656-1790363 [Pseudomonas synxantha]
MTYSLDFDARALKEWQKLGDTLRQQLKKKLMEILNNPRIEANRLHGLPDCYKIKLRSSGYRLVYQVIDQEVTVFVVAIDK
RERDQAYRKASERLDQR

Nucleotide

• Length: 294 bp

>T100212 NZ_CP027756:c1790656-1790363 [Pseudomonas synxantha]
ATGACCTATAGCCTTGATTTTGATGCGCGTGCATTGAAGGAATGGCAGAAACTCGGCGACACCCTCCGTCAGCAACTCAA
GAAGAAGCTGATGGAAATCCTGAACAACCCACGTATTGAGGCTAACCGCCTACATGGCCTGCCTGACTGCTACAAGATCA
AACTGCGTAGTAGCGGGTACAGGCTGGTCTATCAAGTGATAGATCAGGAAGTGACCGTGTTCGTGGTCGCTATTGATAAA
CGTGAACGAGATCAGGCTTATCGCAAAGCGTCTGAGCGCCTTGATCAGCGCTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8997.40 Da
• Isoelectric Point: 4.6017

>AT100212 WP_014717442.1 NZ_CP027756:c1790885-1790646 [Pseudomonas synxantha]
MASINIRIDDDLKARAYLELEKLGVTPSELIRQALQYVAERGQLPFKPVLMTEEDEALMATVRERLASPQRVRVSLDDL

Nucleotide

• Length: 240 bp

>AT100212 NZ_CP027756:c1790885-1790646 [Pseudomonas synxantha]
TTGGCATCTATCAACATCCGTATCGATGACGACCTGAAAGCTCGCGCCTACCTTGAGTTGGAGAAACTCGGTGTCACCCC
TTCAGAACTGATACGTCAGGCGTTGCAATATGTTGCCGAACGCGGCCAGTTGCCATTTAAGCCTGTCCTCATGACTGAAG
AGGATGAAGCCTTGATGGCGACCGTACGTGAGCGCCTTGCGTCGCCTCAACGCGTGAGGGTTTCTTTGGATGACCTATAG

Structures

Antitoxin

• AlphaFold DB: A0A0W0N4Z1