Detailed information of TA system TA100211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1748698..1749313
• Replicon: chromosome
• Accession: NZ_CP027756
• Organism: Pseudomonas synxantha strain R6-28-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K01_RS08085
• Protein ID: WP_082636614.1
• Coordinates: 1749131..1749313 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0R2Z981
• Locus tag: C4K01_RS08080
• Protein ID: WP_057022010.1
• Coordinates: 1748698..1749108 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K01_RS08035	1743895..1744179	-	285	WP_082636613.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
C4K01_RS08040	1744169..1744408	-	240	WP_057022003.1	ribbon-helix-helix protein, CopG family	-
C4K01_RS08045	1744508..1744924	+	417	WP_057022004.1	fosfomycin resistance glutathione transferase	-
C4K01_RS08050	1744972..1745613	+	642	WP_057022005.1	LysE family translocator	-
C4K01_RS08055	1745734..1746261	+	528	WP_005785958.1	DUF4142 domain-containing protein	-
C4K01_RS08060	1746317..1746742	-	426	WP_057022006.1	low affinity iron permease family protein	-
C4K01_RS08065	1746921..1747703	+	783	WP_057022007.1	GNAT family N-acetyltransferase	-
C4K01_RS08070	1747704..1748204	-	501	WP_057022008.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
C4K01_RS08075	1748304..1748615	-	312	WP_057022009.1	hypothetical protein	-
C4K01_RS08080	1748698..1749108	-	411	WP_057022010.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K01_RS08085	1749131..1749313	-	183	WP_082636614.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K01_RS08090	1749480..1750373	-	894	WP_057022012.1	LysR family transcriptional regulator	-
C4K01_RS08095	1750469..1751644	+	1176	WP_057022013.1	MFS transporter	-
C4K01_RS08105	1751965..1752849	-	885	WP_057022014.1	alpha/beta hydrolase	-
C4K01_RS08110	1753151..1753843	-	693	WP_057022015.1	pseudouridine synthase	-
C4K01_RS08115	1753833..1754045	-	213	WP_057022016.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1744169..1761642	17473

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6832.02 Da
• Isoelectric Point: 10.8600

>T100211 WP_082636614.1 NZ_CP027756:c1749313-1749131 [Pseudomonas synxantha]
VDSRYLIGHIVADGWYLVRIRGSHHHFKHPTKPGLVTIPHPKKDLLDKTAKSILRQALLS

Nucleotide

• Length: 183 bp

>T100211 NZ_CP027756:c1749313-1749131 [Pseudomonas synxantha]
GTGGACAGTCGATATTTGATCGGTCATATCGTTGCAGACGGTTGGTATCTTGTACGCATCAGGGGCAGTCATCACCATTT
CAAGCACCCGACGAAGCCAGGGTTGGTAACGATCCCTCATCCAAAAAAGGACCTGCTGGATAAGACCGCCAAGAGCATTT
TGAGACAGGCTCTGTTGAGTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14782.73 Da
• Isoelectric Point: 4.4720

>AT100211 WP_057022010.1 NZ_CP027756:c1749108-1748698 [Pseudomonas synxantha]
MLYPIAISTGDKDHAWGVEVPDIPGCYSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPSAQKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLNHPEEKSRSGFLASAALKVLQQDR

Nucleotide

• Length: 411 bp

>AT100211 NZ_CP027756:c1749108-1748698 [Pseudomonas synxantha]
ATGCTTTACCCCATCGCAATTTCAACCGGCGACAAGGACCACGCGTGGGGCGTGGAGGTCCCGGATATTCCAGGCTGCTA
CTCCGCCGGCGATGACCTGGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATCCTGGCAGAGG
ATGGCGCGCCGATCCCCAGCGCCCAGAAAGTCACCCTGCATGCCGCCAACCCACAGTACGCCGGCTGCACGTGGGCAGTG
GTGGACATCGATGTCACCAAGTACCTGGGCAAGGCGCAAAAACTCAACATCACGCTGCCGGGTTACCTGTTGAATCGCAT
CGATGAATATGTGTTGAATCATCCGGAAGAAAAAAGCCGCTCGGGGTTCCTGGCGTCGGCAGCGCTCAAGGTATTGCAGC
AGGACCGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0R2Z981