Detailed information of TA system TA100209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1042649..1043190
• Replicon: chromosome
• Accession: NZ_CP027756
• Organism: Pseudomonas synxantha strain R6-28-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K01_RS04705
• Protein ID: WP_082636603.1
• Coordinates: 1042915..1043190 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A921NKV7
• Locus tag: C4K01_RS04700
• Protein ID: WP_032893597.1
• Coordinates: 1042649..1042915 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K01_RS04685	1038574..1040859	-	2286	WP_057021591.1	TonB-dependent siderophore receptor	-
C4K01_RS04690	1041065..1041745	+	681	WP_057021590.1	Fe2+-dependent dioxygenase	-
C4K01_RS04695	1041747..1042505	+	759	WP_057021589.1	sel1 repeat family protein	-
C4K01_RS04700	1042649..1042915	+	267	WP_032893597.1	ribbon-helix-helix domain-containing protein	Antitoxin
C4K01_RS04705	1042915..1043190	+	276	WP_082636603.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K01_RS04710	1043254..1044417	-	1164	WP_003188470.1	type III PLP-dependent enzyme	-
C4K01_RS04715	1044888..1046045	-	1158	WP_043048518.1	ABC transporter ATP-binding protein	-
C4K01_RS04720	1046042..1046695	-	654	WP_034118069.1	ABC transporter permease	-
C4K01_RS04725	1046708..1047601	-	894	WP_057021588.1	glycine betaine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10785.36 Da
• Isoelectric Point: 8.5145

>T100209 WP_082636603.1 NZ_CP027756:1042915-1043190 [Pseudomonas synxantha]
MELRWTSKALSDLARLYDFLSVVNREAAARIVQSLSQAPDALLGNPRIGERIEEFSPRDVRRLLVGHYEMRYEIQQSTLY
ILRLWHVREDR

Nucleotide

• Length: 276 bp

>T100209 NZ_CP027756:1042915-1043190 [Pseudomonas synxantha]
ATGGAGCTGAGGTGGACAAGTAAAGCGTTATCCGACCTGGCTCGATTATATGACTTCCTCTCTGTTGTAAACCGCGAAGC
TGCCGCCCGCATCGTGCAGTCACTGTCTCAAGCGCCTGACGCGCTGCTCGGTAATCCGCGCATCGGTGAGAGAATTGAAG
AGTTTTCACCGCGCGACGTAAGAAGACTTTTAGTCGGCCATTACGAAATGCGCTACGAAATTCAACAGTCGACACTTTAT
ATTCTACGGTTATGGCACGTTCGCGAAGATCGTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9728.00 Da
• Isoelectric Point: 4.7640

>AT100209 WP_032893597.1 NZ_CP027756:1042649-1042915 [Pseudomonas synxantha]
MGAETKVMTAHIPTALAQKVDQMAARMDRSRGWVVKQALADWVDQEEERSRLTLEAMADVDAGRVIDHQAVQAWADSLST
DSPLPVPR

Nucleotide

• Length: 267 bp

>AT100209 NZ_CP027756:1042649-1042915 [Pseudomonas synxantha]
ATGGGTGCCGAAACCAAAGTAATGACAGCTCATATTCCCACTGCCCTTGCTCAGAAAGTTGACCAGATGGCCGCGCGAAT
GGATCGCTCACGGGGATGGGTGGTGAAACAGGCCCTCGCTGATTGGGTCGATCAAGAAGAGGAGCGCAGCCGGCTGACGC
TTGAGGCTATGGCAGATGTAGATGCGGGGCGCGTGATCGATCACCAGGCAGTGCAGGCCTGGGCAGATAGCCTGAGCACC
GATTCGCCGCTACCGGTACCGCGCTGA