Detailed information of TA system TA100208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 4923571..4924211
• Replicon: chromosome
• Accession: NZ_CP027755
• Organism: Pseudomonas synxantha strain 2-79

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: C4K02_RS22545
• Protein ID: WP_043050338.1
• Coordinates: 4923571..4923984 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: C4K02_RS22550
• Protein ID: WP_043050337.1
• Coordinates: 4923981..4924211 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K02_RS22530	4919346..4920131	-	786	WP_043050341.1	ABC transporter substrate-binding protein	-
C4K02_RS22535	4920791..4922746	-	1956	WP_043050340.1	acetate--CoA ligase	-
C4K02_RS22540	4923144..4923413	+	270	WP_043050339.1	DUF2790 domain-containing protein	-
C4K02_RS22545	4923571..4923984	-	414	WP_043050338.1	type II toxin-antitoxin system VapC family toxin	Toxin
C4K02_RS22550	4923981..4924211	-	231	WP_043050337.1	virulence factor	Antitoxin
C4K02_RS22555	4924690..4925940	+	1251	WP_057023889.1	ribonucleotide-diphosphate reductase subunit beta	-
C4K02_RS22560	4926015..4926176	+	162	Protein_4331	HNH endonuclease	-
C4K02_RS22565	4926249..4926896	+	648	WP_034128477.1	HAD-IB family hydrolase	-
C4K02_RS22570	4926995..4927954	-	960	WP_043051157.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15127.45 Da
• Isoelectric Point: 7.1890

>T100208 WP_043050338.1 NZ_CP027755:c4923984-4923571 [Pseudomonas synxantha]
VTTYMLDTCICSFIMRERPECVLERLALEVARSNRIVISAITYAEMRYGQIGKKASPKHKTLVDEFVKRLDEVLPWGLAA
VDATVEVKRSLTGQGSIIGQNDTAIAGHAIAAGCTLVTNNVREFSRVAGLSYEDWVH

Nucleotide

• Length: 414 bp

>T100208 NZ_CP027755:c4923984-4923571 [Pseudomonas synxantha]
GTGACGACATACATGCTGGATACCTGCATTTGCTCATTCATCATGCGTGAACGGCCCGAGTGTGTGCTTGAGCGACTGGC
ATTGGAGGTCGCGAGGAGCAATCGAATCGTGATTTCGGCCATAACCTATGCCGAAATGCGATATGGACAGATTGGGAAAA
AGGCTTCGCCCAAACACAAGACGTTAGTCGATGAGTTTGTGAAGCGGCTCGATGAGGTATTGCCTTGGGGCTTGGCAGCG
GTGGACGCGACGGTTGAGGTGAAGCGAAGCTTGACTGGGCAAGGGTCGATCATCGGTCAAAATGACACGGCGATAGCCGG
GCATGCAATTGCTGCGGGATGTACGTTGGTAACCAACAACGTCCGGGAATTCAGTCGTGTAGCGGGGTTGTCTTACGAGG
ACTGGGTTCACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8709.77 Da
• Isoelectric Point: 4.7719

>AT100208 WP_043050337.1 NZ_CP027755:c4924211-4923981 [Pseudomonas synxantha]
MRTVSIFKNGKNQAIRFPNDMAYEGVGELEITRNGDTITLRPVRPSWGSLRELPKADEDFLQERQDVIGDEGRFNL

Nucleotide

• Length: 231 bp

>AT100208 NZ_CP027755:c4924211-4923981 [Pseudomonas synxantha]
ATGCGCACAGTCTCGATTTTTAAAAACGGAAAGAACCAGGCTATTCGATTCCCAAACGACATGGCCTATGAGGGAGTCGG
CGAACTCGAAATCACCCGCAACGGAGATACGATTACGTTGCGACCTGTACGACCTAGCTGGGGGTCGTTGCGTGAACTGC
CCAAGGCGGACGAAGATTTTCTACAGGAGCGTCAGGACGTTATTGGCGATGAAGGCAGGTTCAATCTGTGA