Detailed information of TA system TA100204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 974157..974698
• Replicon: chromosome
• Accession: NZ_CP027755
• Organism: Pseudomonas synxantha strain 2-79

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K02_RS04380
• Protein ID: WP_080889050.1
• Coordinates: 974423..974698 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A921NKV7
• Locus tag: C4K02_RS04375
• Protein ID: WP_032893597.1
• Coordinates: 974157..974423 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K02_RS04360	970082..972367	-	2286	WP_043048513.1	TonB-dependent siderophore receptor	-
C4K02_RS04365	972573..973253	+	681	WP_043048515.1	Fe2+-dependent dioxygenase	-
C4K02_RS04370	973255..974013	+	759	WP_043048516.1	sel1 repeat family protein	-
C4K02_RS04375	974157..974423	+	267	WP_032893597.1	ribbon-helix-helix domain-containing protein	Antitoxin
C4K02_RS04380	974423..974698	+	276	WP_080889050.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K02_RS04385	974762..975925	-	1164	WP_003188470.1	type III PLP-dependent enzyme	-
C4K02_RS04390	976400..977557	-	1158	WP_043048518.1	ABC transporter ATP-binding protein	-
C4K02_RS04395	977554..978207	-	654	WP_034118069.1	ABC transporter permease	-
C4K02_RS04400	978220..979113	-	894	WP_043048519.1	glycine betaine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10770.39 Da
• Isoelectric Point: 9.9718

>T100204 WP_080889050.1 NZ_CP027755:974423-974698 [Pseudomonas synxantha]
MELRWTSKALSDLARLYDFLSVVNREAAARVVQSLSQAPDALLGNPRIGERIEEFSPRDVRRLLVGHYEMRYEIQQSTLY
ILRLWHVRKDR

Nucleotide

• Length: 276 bp

>T100204 NZ_CP027755:974423-974698 [Pseudomonas synxantha]
ATGGAGCTGAGGTGGACAAGTAAAGCCTTATCCGACCTGGCTCGATTATATGACTTCCTCTCTGTTGTAAACCGCGAAGC
TGCCGCCCGCGTCGTGCAGTCACTGTCTCAAGCGCCTGACGCGCTGCTCGGTAATCCGCGCATCGGTGAGAGAATTGAAG
AGTTTTCACCGCGCGACGTAAGAAGACTTTTAGTCGGCCATTACGAAATGCGCTACGAAATTCAACAGTCGACACTTTAT
ATTCTACGGTTATGGCACGTTCGCAAAGATCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9728.00 Da
• Isoelectric Point: 4.7640

>AT100204 WP_032893597.1 NZ_CP027755:974157-974423 [Pseudomonas synxantha]
MGAETKVMTAHIPTALAQKVDQMAARMDRSRGWVVKQALADWVDQEEERSRLTLEAMADVDAGRVIDHQAVQAWADSLST
DSPLPVPR

Nucleotide

• Length: 267 bp

>AT100204 NZ_CP027755:974157-974423 [Pseudomonas synxantha]
ATGGGTGCCGAAACCAAAGTAATGACAGCTCATATTCCCACTGCCCTTGCTCAGAAAGTTGACCAGATGGCCGCGCGAAT
GGATCGCTCACGGGGATGGGTGGTGAAACAGGCCCTCGCTGATTGGGTCGATCAAGAAGAAGAACGTAGCCGTTTGACGC
TTGAGGCGATGGCAGATGTGGATGCGGGGCGCGTGATTGATCACCAGGCAGTGCAAGCCTGGGCCGACAGCCTGAGTACG
GATTCGCCGCTACCGGTACCGCGCTGA