Detailed information of TA system TA100198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1797931..1798546
• Replicon: chromosome
• Accession: NZ_CP027754
• Organism: Pseudomonas synxantha strain 30B

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I4KFU3
• Locus tag: C4K03_RS08525
• Protein ID: WP_003189411.1
• Coordinates: 1798364..1798546 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K03_RS08520
• Protein ID: WP_124376845.1
• Coordinates: 1797931..1798341 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K03_RS08475	1793126..1793410	-	285	WP_124376836.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4K03_RS08480	1793400..1793639	-	240	WP_124376837.1	ribbon-helix-helix protein, CopG family	-
C4K03_RS08485	1793739..1794155	+	417	WP_124376838.1	fosfomycin resistance glutathione transferase	-
C4K03_RS08490	1794203..1794844	+	642	WP_124376839.1	LysE family translocator	-
C4K03_RS08495	1794965..1795492	+	528	WP_124376840.1	DUF4142 domain-containing protein	-
C4K03_RS08500	1795548..1795973	-	426	WP_124376841.1	low affinity iron permease family protein	-
C4K03_RS08505	1796154..1796936	+	783	WP_124376842.1	GNAT family N-acetyltransferase	-
C4K03_RS08510	1796937..1797437	-	501	WP_124376843.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
C4K03_RS08515	1797537..1797848	-	312	WP_124376844.1	hypothetical protein	-
C4K03_RS08520	1797931..1798341	-	411	WP_124376845.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K03_RS08525	1798364..1798546	-	183	WP_003189411.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K03_RS08530	1798713..1799606	-	894	WP_124376846.1	LysR family transcriptional regulator	-
C4K03_RS08535	1799702..1800871	+	1170	WP_124376847.1	MFS transporter	-
C4K03_RS08545	1801192..1802076	-	885	WP_124376848.1	alpha/beta hydrolase	-
C4K03_RS08550	1802383..1803075	-	693	WP_124376849.1	pseudouridine synthase	-
C4K03_RS08555	1803065..1803277	-	213	WP_124376850.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6804.01 Da
• Isoelectric Point: 10.7532

>T100198 WP_003189411.1 NZ_CP027754:c1798546-1798364 [Pseudomonas synxantha]
VDSRYLIGHIVADGWYLVRIRGSHHHFKHPTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Nucleotide

• Length: 183 bp

>T100198 NZ_CP027754:c1798546-1798364 [Pseudomonas synxantha]
GTGGACAGCCGATATTTGATCGGTCATATCGTTGCAGACGGTTGGTATCTTGTACGCATCAGGGGCAGTCATCACCATTT
CAAGCACCCGACGAAGCCAGGGTTGGTAACGATCCCTCATCCAAAAAAGGACCTGCTGGATAAGACCGCAAAGAGCATCT
TGAAACAGGCTCTGTTGAGTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14855.82 Da
• Isoelectric Point: 4.4720

>AT100198 WP_124376845.1 NZ_CP027754:c1798341-1797931 [Pseudomonas synxantha]
MLYPIAISTGDKDHAWGVEVPDIPGCYSAGDDLDDAMAMAREAIEGHFEILAEDGAQIPSAQKVTLHAVNPQYAGCTWAV
VDIDITKYLGKAQKLNITLPGYLLNRIDEYVLNHPEEKSRSGFLASAALKVLQQDR

Nucleotide

• Length: 411 bp

>AT100198 NZ_CP027754:c1798341-1797931 [Pseudomonas synxantha]
ATGCTTTACCCCATCGCAATTTCAACCGGTGACAAGGACCACGCGTGGGGCGTGGAGGTCCCGGATATTCCAGGTTGCTA
CTCCGCCGGCGATGACCTGGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATCCTGGCAGAGG
ATGGCGCGCAGATCCCCAGCGCCCAGAAAGTCACCCTGCATGCCGTCAACCCACAGTACGCCGGCTGCACGTGGGCAGTG
GTGGACATCGATATCACCAAGTACCTGGGCAAGGCGCAAAAACTCAACATCACCCTGCCGGGGTACCTGTTGAATCGCAT
CGATGAATATGTGCTCAATCATCCGGAAGAAAAAAGCCGCTCGGGGTTCCTGGCGTCGGCAGCGCTCAAGGTATTGCAGC
AGGACCGGTAA

Structures

Toxin

• AlphaFold DB: A0A1X7DFI9