Detailed information of TA system TA100192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2594662..2595247
• Replicon: chromosome
• Accession: NZ_CP027753
• Organism: Pseudomonas chlororaphis strain B25

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K04_RS12050
• Protein ID: WP_124320163.1
• Coordinates: 2594954..2595247 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C4K04_RS12045
• Protein ID: WP_124320162.1
• Coordinates: 2594662..2594952 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K04_RS12030	2590007..2592487	+	2481	WP_124320159.1	FtsX-like permease family protein	-
C4K04_RS12035	2592477..2593559	+	1083	WP_124320160.1	iron ABC transporter permease	-
C4K04_RS12040	2593674..2594261	+	588	WP_124320161.1	hypothetical protein	-
C4K04_RS12045	2594662..2594952	-	291	WP_124320162.1	putative addiction module antidote protein	Antitoxin
C4K04_RS12050	2594954..2595247	-	294	WP_124320163.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K04_RS12055	2595483..2596877	-	1395	WP_124320164.1	VOC family protein	-
C4K04_RS12060	2597141..2598265	+	1125	WP_124320165.1	diguanylate cyclase	-
C4K04_RS12065	2598359..2599603	+	1245	WP_124320166.1	M20/M25/M40 family metallo-hydrolase	-
C4K04_RS12070	2599840..2600238	+	399	WP_124320167.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (10-94)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10726.35 Da
• Isoelectric Point: 10.7340

>T100192 WP_124320163.1 NZ_CP027753:c2595247-2594954 [Pseudomonas chlororaphis]
MDYEILQTTVFARWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSQMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T100192 NZ_CP027753:c2595247-2594954 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCGATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGTGGTCTCTCGCAGATGCGCG
TGAATACAGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCAGCCAGCCTGCCGATATCTGCCAGGCTCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10152.65 Da
• Isoelectric Point: 6.9748

>AT100192 WP_124320162.1 NZ_CP027753:c2594952-2594662 [Pseudomonas chlororaphis]
MSDTLVPFDIAALLDGDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIATATGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVSRG

Nucleotide

• Length: 291 bp

>AT100192 NZ_CP027753:c2594952-2594662 [Pseudomonas chlororaphis]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCGCCCTGCTCGACGGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
CGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAACTGTGCACCACCGTGTCCCGAGGCTGA