Detailed information of TA system TA100191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1623059..1623681
• Replicon: chromosome
• Accession: NZ_CP027753
• Organism: Pseudomonas chlororaphis strain B25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K04_RS07540
• Protein ID: WP_124319561.1
• Coordinates: 1623499..1623681 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K04_RS07535
• Protein ID: WP_124323007.1
• Coordinates: 1623059..1623466 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K04_RS07510	1618059..1618721	-	663	WP_124319558.1	NAD(P)-binding domain-containing protein	-
C4K04_RS07515	1618821..1619714	+	894	Protein_1464	LysR family transcriptional regulator	-
C4K04_RS07520	1619953..1621731	+	1779	WP_124319559.1	EAL domain-containing protein	-
C4K04_RS07525	1621765..1622679	-	915	WP_124319560.1	SGNH/GDSL hydrolase family protein	-
C4K04_RS07530	1622758..1622988	-	231	Protein_1467	hypothetical protein	-
C4K04_RS07535	1623059..1623466	-	408	WP_124323007.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K04_RS07540	1623499..1623681	-	183	WP_124319561.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K04_RS07545	1624551..1625087	-	537	WP_124319562.1	helix-turn-helix domain-containing protein	-
C4K04_RS07550	1625227..1625964	+	738	WP_124319563.1	metallophosphoesterase	-
C4K04_RS07555	1626054..1626404	+	351	WP_206429791.1	hypothetical protein	-
C4K04_RS07560	1626414..1626818	+	405	WP_124319564.1	hypothetical protein	-
C4K04_RS07565	1626867..1627223	+	357	WP_124319565.1	hypothetical protein	-
C4K04_RS07570	1628176..1628450	+	275	Protein_1475	DUF4113 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1623059..1638915	15856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6872.20 Da
• Isoelectric Point: 11.4018

>T100191 WP_124319561.1 NZ_CP027753:c1623681-1623499 [Pseudomonas chlororaphis]
VNSRYLIGQIVADGWYLVRVRGSHHHFKHPHKPGLVTVPHPKKDLLRKTAISILKQALLM

Nucleotide

• Length: 183 bp

>T100191 NZ_CP027753:c1623681-1623499 [Pseudomonas chlororaphis]
GTGAACAGTCGGTATTTGATCGGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGGGGCAGTCACCACCATTT
CAAGCATCCCCATAAGCCGGGGCTGGTGACCGTTCCCCATCCCAAAAAGGACCTGCTGAGAAAAACCGCCATCAGCATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14644.67 Da
• Isoelectric Point: 4.4709

>AT100191 WP_124323007.1 NZ_CP027753:c1623466-1623059 [Pseudomonas chlororaphis]
MLYPVAISMGDDEHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPPANKVTLHAANPRYAGCTWAL
IDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEAKSRSGFLASAALKVLQQD

Nucleotide

• Length: 408 bp

>AT100191 NZ_CP027753:c1623466-1623059 [Pseudomonas chlororaphis]
ATGCTTTACCCGGTTGCAATTTCAATGGGCGATGACGAACACGCCTGGGGCGTGGAAGTCCCCGACATTCCTGGCTGTTT
CTCGGCCGGCGACGACCTCGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATTCTCGCCGAAG
ACGGTGCCCCCATCCCGCCCGCCAACAAGGTCACCCTGCACGCGGCCAACCCCCGGTACGCAGGCTGCACCTGGGCGCTG
ATCGACATCGATGTCACCAAGTACCTGGGCAAGGCGCAGAAGCTCAATATCACCCTGCCCGGCTACCTGCTGAACCGCAT
CGACGAATACGTGCTGCATCACCCGGAAGCCAAGAGCCGCTCGGGTTTTCTCGCTTCAGCTGCACTGAAGGTATTGCAGC
AAGACTGA