Detailed information of TA system TA100189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 318507..319120
• Replicon: chromosome
• Accession: NZ_CP027753
• Organism: Pseudomonas chlororaphis strain B25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K04_RS01415
• Protein ID: WP_124318710.1
• Coordinates: 318507..318689 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2N8BII0
• Locus tag: C4K04_RS01420
• Protein ID: WP_009041686.1
• Coordinates: 318719..319120 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K04_RS01395	314127..316091	+	1965	WP_164486541.1	choline transporter BetT	-
C4K04_RS01400	316174..316431	-	258	WP_124318707.1	hypothetical protein	-
C4K04_RS01405	316611..317351	-	741	WP_124318708.1	SDR family oxidoreductase	-
C4K04_RS01410	317492..318382	+	891	WP_124318709.1	LysR family transcriptional regulator	-
C4K04_RS01415	318507..318689	+	183	WP_124318710.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K04_RS01420	318719..319120	+	402	WP_009041686.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K04_RS01425	319707..320744	+	1038	WP_124318711.1	L-glyceraldehyde 3-phosphate reductase	-
C4K04_RS01430	320870..321298	-	429	WP_124318712.1	type II toxin-antitoxin system VapC family toxin	-
C4K04_RS01435	321295..321540	-	246	WP_124318713.1	plasmid stabilization protein	-
C4K04_RS01440	321668..322507	-	840	WP_124318714.1	taurine dioxygenase	-
C4K04_RS01445	322745..323584	-	840	WP_124318715.1	taurine ABC transporter permease TauC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6947.11 Da
• Isoelectric Point: 10.4588

>T100189 WP_124318710.1 NZ_CP027753:318507-318689 [Pseudomonas chlororaphis]
MRSRELIRMIEEDGWYLIAIKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Nucleotide

• Length: 183 bp

>T100189 NZ_CP027753:318507-318689 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGATCAAAGGCAGTCATCACCAGTA
CAAGCATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14629.48 Da
• Isoelectric Point: 4.7569

>AT100189 WP_009041686.1 NZ_CP027753:318719-319120 [Pseudomonas chlororaphis]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAEAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGMI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT100189 NZ_CP027753:318719-319120 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCGGCAGAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAACCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGCATGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGCTTCAACGCCACCCTGCCGGAGCAATTGCTGGAACGCATCGA
CCAGACAGTGAAGCGCGACCAGCGTTACCGCTCGCGTTCGGGCTTTCTCGCGGCTGCCGCTTTACGCGAACTCTCCGCCT
AG

Structures

Antitoxin

• AlphaFold DB: A0A2N8BII0