Detailed information of TA system TA100180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 201864..202380
• Replicon: chromosome
• Accession: NZ_CP027752
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzTR38

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: C4K05_RS00865
• Protein ID: WP_124301008.1
• Coordinates: 202099..202380 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A554PKE4
• Locus tag: C4K05_RS00860
• Protein ID: WP_009046356.1
• Coordinates: 201864..202109 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K05_RS00835	197632..198627	+	996	WP_124312600.1	sulfotransferase family protein	-
C4K05_RS00840	198593..199351	-	759	WP_009046352.1	slipin family protein	-
C4K05_RS00845	199353..200738	-	1386	WP_009046353.1	nodulation protein NfeD	-
C4K05_RS00850	200962..201423	+	462	WP_016705004.1	YbaK/EbsC family protein	-
C4K05_RS00855	201554..201799	+	246	WP_009046355.1	DUF2789 domain-containing protein	-
C4K05_RS00860	201864..202109	+	246	WP_009046356.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4K05_RS00865	202099..202380	+	282	WP_124301008.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K05_RS00870	202513..202941	+	429	WP_009046358.1	winged helix DNA-binding protein	-
C4K05_RS00875	202938..205022	+	2085	WP_016705005.1	FUSC family protein	-
C4K05_RS00880	205019..205228	+	210	WP_007932080.1	DUF1656 domain-containing protein	-
C4K05_RS00885	205225..206121	+	897	WP_124301009.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10933.86 Da
• Isoelectric Point: 10.6258

>T100180 WP_124301008.1 NZ_CP027752:202099-202380 [Pseudomonas chlororaphis subsp. aureofaciens]
MTYKLQFLPSARKEWDKLGHTLREQFKRKLAERLEMPRVPADALHGMADCYKIKLKPSGYRLVYQVIEDQVVVSVVAVGK
RERSAVYERARKR

Nucleotide

• Length: 282 bp

>T100180 NZ_CP027752:202099-202380 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGACTTATAAGCTCCAGTTCCTGCCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTCATACCCTGCGCGAGCAATTCAA
GAGGAAGCTGGCCGAGCGCCTGGAAATGCCGCGGGTGCCCGCGGACGCCCTTCACGGTATGGCGGACTGCTACAAGATCA
AGCTCAAGCCCTCTGGCTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTCGTGGTTTCGGTGGTGGCCGTGGGCAAA
CGTGAACGCAGTGCCGTGTATGAGCGGGCCCGAAAGCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8814.14 Da
• Isoelectric Point: 4.7164

>AT100180 WP_009046356.1 NZ_CP027752:201864-202109 [Pseudomonas chlororaphis subsp. aureofaciens]
MQSVLADKAVSVSELKKNPSAVLNGAQGGAVAVLNHNRVMGYMVPADVFEAMMERLDDLELAEIVRARRHETPVPVNLDD
L

Nucleotide

• Length: 246 bp

>AT100180 NZ_CP027752:201864-202109 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAATCCTTCGGCTGTCCTGAACGGCGCACA
GGGTGGTGCGGTTGCGGTGCTCAATCACAACCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCCATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGTCACGAGACCCCGGTCCCGGTAAATCTGGATGAC
TTATAA

Structures

Antitoxin

• AlphaFold DB: A0A554PKE4