Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 2352158..2352743 Replicon chromosome
Accession NZ_CP027751
Organism Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzTR18

Toxin (Protein)


Gene name PumA Uniprot ID A0A7S5HMS4
Locus tag C4K06_RS10775 Protein ID WP_016705075.1
Coordinates 2352450..2352743 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A8A9ZIA4
Locus tag C4K06_RS10770 Protein ID WP_009048177.1
Coordinates 2352158..2352448 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K06_RS10760 2349670..2351052 + 1383 WP_009048175.1 efflux transporter outer membrane subunit -
C4K06_RS10765 2351171..2351758 + 588 WP_028682910.1 hypothetical protein -
C4K06_RS10770 2352158..2352448 - 291 WP_009048177.1 putative addiction module antidote protein Antitoxin
C4K06_RS10775 2352450..2352743 - 294 WP_016705075.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C4K06_RS10780 2352978..2354372 - 1395 WP_053268635.1 VOC family protein -
C4K06_RS10785 2354637..2355761 + 1125 WP_041988396.1 diguanylate cyclase -
C4K06_RS10790 2356034..2356459 - 426 WP_124303536.1 tail fiber assembly protein -
C4K06_RS10795 2356473..2357540 - 1068 WP_124303538.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2352158..2391149 38991


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-97)

Antitoxin

Uncharacterized protein HI1420

(9-91)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10699.27 Da        Isoelectric Point: 10.0959

>T100177 WP_016705075.1 NZ_CP027751:c2352743-2352450 [Pseudomonas chlororaphis subsp. aureofaciens]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Download         Length: 294 bp

>T100177 NZ_CP027751:c2352743-2352450 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCGTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATACAGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCAGCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10279.84 Da        Isoelectric Point: 8.0096

>AT100177 WP_009048177.1 NZ_CP027751:c2352448-2352158 [Pseudomonas chlororaphis subsp. aureofaciens]
MSDTLVPFDIATLLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVPRG

Download         Length: 291 bp

>AT100177 NZ_CP027751:c2352448-2352158 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGCCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGCCCCGAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7S5HMS4

Antitoxin

Source ID Structure
AlphaFold DB A0A8A9ZIA4

References