Detailed information of TA system TA100176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2323936..2324555
• Replicon: chromosome
• Accession: NZ_CP027751
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzTR18

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K06_RS10650
• Protein ID: WP_124301294.1
• Coordinates: 2324373..2324555 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K06_RS10645
• Protein ID: WP_092418556.1
• Coordinates: 2323936..2324337 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K06_RS10630	2320267..2320902	+	636	WP_124301293.1	response regulator transcription factor	-
C4K06_RS10635	2320996..2321760	+	765	WP_051423542.1	hypothetical protein	-
C4K06_RS10640	2322019..2323320	+	1302	WP_080710636.1	IS4 family transposase	-
C4K06_RS10645	2323936..2324337	-	402	WP_092418556.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K06_RS10650	2324373..2324555	-	183	WP_124301294.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K06_RS10655	2324920..2325201	-	282	WP_053268620.1	hypothetical protein	-
C4K06_RS10660	2325718..2326878	-	1161	WP_053275419.1	IS30 family transposase	-
C4K06_RS10665	2327359..2327646	+	288	WP_124301295.1	hypothetical protein	-
C4K06_RS10670	2327691..2328821	+	1131	WP_124301296.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2316576..2340439	23863
	-	inside	IScluster/Tn	-	-	2321968..2326878	4910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6827.99 Da
• Isoelectric Point: 11.1724

>T100176 WP_124301294.1 NZ_CP027751:c2324555-2324373 [Pseudomonas chlororaphis subsp. aureofaciens]
VQSRQLIKELEAAGWVLERVTGSHHLFKHPYRPETVPVPHPRKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 183 bp

>T100176 NZ_CP027751:c2324555-2324373 [Pseudomonas chlororaphis subsp. aureofaciens]
GTGCAAAGCAGACAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CAAGCATCCCTACCGGCCCGAGACCGTTCCGGTGCCTCACCCGAGGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14588.55 Da
• Isoelectric Point: 4.7465

>AT100176 WP_092418556.1 NZ_CP027751:c2324337-2323936 [Pseudomonas chlororaphis subsp. aureofaciens]
VHYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELAREGQAIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT100176 NZ_CP027751:c2324337-2323936 [Pseudomonas chlororaphis subsp. aureofaciens]
GTGCACTATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGTG
AGGGACAAGCCATTCCCCTGCCCTCGCCCACCGGCACACACCGCGCCAACCCCGACTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGACATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTGCGGGAGCACAACATCAAGAGCCGGTCGTCTTTTCTGGCAGATGCGGCGATGGAAAAGCTGGGGCGTT
AA