Detailed information of TA system TA100173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 201861..202377
• Replicon: chromosome
• Accession: NZ_CP027751
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzTR18

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: C4K06_RS00865
• Protein ID: WP_124301008.1
• Coordinates: 202096..202377 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A554PKE4
• Locus tag: C4K06_RS00860
• Protein ID: WP_009046356.1
• Coordinates: 201861..202106 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K06_RS00835	197629..198624	+	996	WP_016705003.1	aspartyl/asparaginyl beta-hydroxylase	-
C4K06_RS00840	198590..199348	-	759	WP_124303339.1	slipin family protein	-
C4K06_RS00845	199350..200735	-	1386	WP_009046353.1	nodulation protein NfeD	-
C4K06_RS00850	200959..201420	+	462	WP_016705004.1	YbaK/EbsC family protein	-
C4K06_RS00855	201551..201796	+	246	WP_009046355.1	DUF2789 domain-containing protein	-
C4K06_RS00860	201861..202106	+	246	WP_009046356.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4K06_RS00865	202096..202377	+	282	WP_124301008.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K06_RS00870	202510..202938	+	429	WP_009046358.1	winged helix DNA-binding protein	-
C4K06_RS00875	202935..205019	+	2085	WP_016705005.1	FUSC family protein	-
C4K06_RS00880	205016..205225	+	210	WP_007932080.1	DUF1656 domain-containing protein	-
C4K06_RS00885	205222..206118	+	897	WP_124301009.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10933.86 Da
• Isoelectric Point: 10.6258

>T100173 WP_124301008.1 NZ_CP027751:202096-202377 [Pseudomonas chlororaphis subsp. aureofaciens]
MTYKLQFLPSARKEWDKLGHTLREQFKRKLAERLEMPRVPADALHGMADCYKIKLKPSGYRLVYQVIEDQVVVSVVAVGK
RERSAVYERARKR

Nucleotide

• Length: 282 bp

>T100173 NZ_CP027751:202096-202377 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGACTTATAAGCTCCAGTTCCTGCCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTCATACCCTGCGCGAGCAATTCAA
GAGGAAGCTGGCCGAGCGCCTGGAAATGCCGCGGGTGCCCGCGGACGCCCTTCACGGTATGGCGGACTGCTACAAGATCA
AGCTCAAGCCCTCTGGCTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTCGTGGTTTCGGTGGTGGCCGTGGGCAAA
CGTGAACGCAGTGCCGTGTATGAGCGGGCCCGAAAGCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8814.14 Da
• Isoelectric Point: 4.7164

>AT100173 WP_009046356.1 NZ_CP027751:201861-202106 [Pseudomonas chlororaphis subsp. aureofaciens]
MQSVLADKAVSVSELKKNPSAVLNGAQGGAVAVLNHNRVMGYMVPADVFEAMMERLDDLELAEIVRARRHETPVPVNLDD
L

Nucleotide

• Length: 246 bp

>AT100173 NZ_CP027751:201861-202106 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAATCCTTCGGCTGTCCTGAACGGCGCACA
GGGTGGTGCGGTTGCGGTGCTCAATCACAACCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCCATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGTCACGAGACCCCGGTCCCGGTAAATCTGGATGAC
TTATAA

Structures

Antitoxin

• AlphaFold DB: A0A554PKE4