Detailed information of TA system TA100165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 201676..202192
• Replicon: chromosome
• Accession: NZ_CP027750
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzS24

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: C4K07_RS00865
• Protein ID: WP_124301008.1
• Coordinates: 201911..202192 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A554PKE4
• Locus tag: C4K07_RS00860
• Protein ID: WP_009046356.1
• Coordinates: 201676..201921 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K07_RS00835	197445..198440	+	996	WP_124301006.1	sulfotransferase family protein	-
C4K07_RS00840	198406..199164	-	759	WP_009046352.1	slipin family protein	-
C4K07_RS00845	199166..200551	-	1386	WP_009046353.1	nodulation protein NfeD	-
C4K07_RS00850	200775..201236	+	462	WP_016705004.1	YbaK/EbsC family protein	-
C4K07_RS00855	201367..201642	+	276	WP_124301007.1	DUF2789 domain-containing protein	-
C4K07_RS00860	201676..201921	+	246	WP_009046356.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4K07_RS00865	201911..202192	+	282	WP_124301008.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K07_RS00870	202325..202753	+	429	WP_009046358.1	winged helix DNA-binding protein	-
C4K07_RS00875	202750..204834	+	2085	WP_016705005.1	FUSC family protein	-
C4K07_RS00880	204831..205040	+	210	WP_007932080.1	DUF1656 domain-containing protein	-
C4K07_RS00885	205037..205933	+	897	WP_124301009.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10933.86 Da
• Isoelectric Point: 10.6258

>T100165 WP_124301008.1 NZ_CP027750:201911-202192 [Pseudomonas chlororaphis subsp. aureofaciens]
MTYKLQFLPSARKEWDKLGHTLREQFKRKLAERLEMPRVPADALHGMADCYKIKLKPSGYRLVYQVIEDQVVVSVVAVGK
RERSAVYERARKR

Nucleotide

• Length: 282 bp

>T100165 NZ_CP027750:201911-202192 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGACTTATAAGCTCCAGTTCCTGCCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTCATACCCTGCGCGAGCAATTCAA
GAGGAAGCTGGCCGAGCGCCTGGAAATGCCGCGGGTGCCCGCGGACGCCCTTCACGGTATGGCGGACTGCTACAAGATCA
AGCTCAAGCCCTCTGGCTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTCGTGGTTTCGGTGGTGGCCGTGGGCAAA
CGTGAACGCAGTGCCGTGTATGAGCGGGCCCGAAAGCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8814.14 Da
• Isoelectric Point: 4.7164

>AT100165 WP_009046356.1 NZ_CP027750:201676-201921 [Pseudomonas chlororaphis subsp. aureofaciens]
MQSVLADKAVSVSELKKNPSAVLNGAQGGAVAVLNHNRVMGYMVPADVFEAMMERLDDLELAEIVRARRHETPVPVNLDD
L

Nucleotide

• Length: 246 bp

>AT100165 NZ_CP027750:201676-201921 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAATCCTTCGGCTGTCCTGAACGGCGCACA
GGGTGGTGCGGTTGCGGTGCTCAATCACAACCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCCATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGTCACGAGACCCCGGTCCCGGTAAATCTGGATGAC
TTATAA

Structures

Antitoxin

• AlphaFold DB: A0A554PKE4