Detailed information of TA system TA100157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 6037472..6038061
• Replicon: chromosome
• Accession: NZ_CP027747
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain 66

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A554PHZ9
• Locus tag: C4K10_RS27370
• Protein ID: WP_041987451.1
• Coordinates: 6037762..6038061 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C4K10_RS27365
• Protein ID: WP_041987449.1
• Coordinates: 6037472..6037765 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K10_RS27345	6033013..6034176	-	1164	WP_124302191.1	acyltransferase	-
C4K10_RS27350	6034371..6035561	-	1191	WP_124340799.1	serine hydrolase	-
C4K10_RS27355	6035688..6036575	+	888	WP_028683471.1	LysR family transcriptional regulator	-
C4K10_RS27360	6036947..6037339	+	393	WP_009051075.1	hypothetical protein	-
C4K10_RS27365	6037472..6037765	-	294	WP_041987449.1	putative addiction module antidote protein	Antitoxin
C4K10_RS27370	6037762..6038061	-	300	WP_041987451.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K10_RS27375	6038232..6038681	+	450	WP_124315435.1	DUF3828 domain-containing protein	-
C4K10_RS27380	6038706..6039716	-	1011	WP_206430875.1	zinc-binding dehydrogenase	-
C4K10_RS27385	6039774..6040073	-	300	WP_009051080.1	ArsR family transcriptional regulator	-
C4K10_RS27390	6040137..6041228	-	1092	WP_009051081.1	nitrite reductase, copper-containing	-
C4K10_RS27395	6041454..6041696	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
C4K10_RS27400	6041693..6042580	+	888	WP_028683466.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11231.97 Da
• Isoelectric Point: 10.6086

>T100157 WP_041987451.1 NZ_CP027747:c6038061-6037762 [Pseudomonas chlororaphis subsp. aureofaciens]
MIEIKQTATFMEWENRLKDSRARALIAARIFRLANGLAGDVPPVGEGVSELRIHYGPGYRVYFQQLGSQLVILLCGGDKS
RQSRDIELAKQLARRWSRS

Nucleotide

• Length: 300 bp

>T100157 NZ_CP027747:c6038061-6037762 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATCGC
TGCGCGGATTTTTCGCCTGGCCAATGGCCTTGCCGGTGACGTGCCACCGGTGGGCGAGGGCGTCAGCGAGTTGCGCATCC
ATTACGGGCCGGGCTATCGGGTTTACTTCCAGCAGCTTGGTTCGCAACTCGTCATCCTGTTATGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAACAGCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10446.94 Da
• Isoelectric Point: 5.6558

>AT100157 WP_041987449.1 NZ_CP027747:c6037765-6037472 [Pseudomonas chlororaphis subsp. aureofaciens]
MNEPLFDYDPATSLRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLDMTARVHGAS

Nucleotide

• Length: 294 bp

>AT100157 NZ_CP027747:c6037765-6037472 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAACGAACCGCTATTCGACTATGACCCCGCCACGTCCCTGCGTGACCCGCAGGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGCATGACCGAACTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTACCGCTCGTTCAGTGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGCGCCCTGGGGCTGGATATGACGGCGCGGGTGCATGGGGCGTCTTGA

Structures

Toxin

• AlphaFold DB: A0A554PHZ9