Detailed information of TA system TA100154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2365155..2365774
• Replicon: chromosome
• Accession: NZ_CP027747
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain 66

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K10_RS10945
• Protein ID: WP_124339877.1
• Coordinates: 2365592..2365774 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K10_RS10940
• Protein ID: WP_124339876.1
• Coordinates: 2365155..2365556 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K10_RS10925	2361035..2362924	+	1890	WP_124339875.1	PAS domain S-box protein	-
C4K10_RS10930	2362921..2363556	+	636	WP_009048153.1	response regulator transcription factor	-
C4K10_RS10935	2363650..2364414	+	765	WP_101282091.1	hypothetical protein	-
C4K10_RS10940	2365155..2365556	-	402	WP_124339876.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K10_RS10945	2365592..2365774	-	183	WP_124339877.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K10_RS10950	2366137..2366418	-	282	WP_124339878.1	hypothetical protein	-
C4K10_RS10955	2366855..2367835	-	981	WP_124339879.1	DUF4238 domain-containing protein	-
C4K10_RS10960	2368036..2369037	+	1002	WP_124339880.1	GIY-YIG nuclease family protein	-
C4K10_RS10965	2369446..2369967	-	522	WP_164485448.1	SLATT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2359230..2378905	19675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6803.97 Da
• Isoelectric Point: 10.9678

>T100154 WP_124339877.1 NZ_CP027747:c2365774-2365592 [Pseudomonas chlororaphis subsp. aureofaciens]
VQNRQLIKELEAAGWVLERVTGSHHLFKNPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 183 bp

>T100154 NZ_CP027747:c2365774-2365592 [Pseudomonas chlororaphis subsp. aureofaciens]
GTGCAAAACAGACAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CAAGAATCCCTACCGGCCGGAGACCGTTCCAGTGCCTCATCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14588.55 Da
• Isoelectric Point: 4.7465

>AT100154 WP_124339876.1 NZ_CP027747:c2365556-2365155 [Pseudomonas chlororaphis subsp. aureofaciens]
VHYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELAREGQTIPLPSPAGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT100154 NZ_CP027747:c2365556-2365155 [Pseudomonas chlororaphis subsp. aureofaciens]
GTGCACTATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGAATCCAGATTCCTGACATTCCTGGGGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTATAGCGCCGCCATCGAGATCGCTCACGTCATGCTTGAAGAGCTGGCCCGTG
AGGGGCAAACCATTCCCCTGCCCTCGCCCGCCGGCACACACCGCGCCAACCCCGATTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGTTTTGTGCGCGAGCACAACATCAAGAGCCGGTCGTCTTTTCTGGCGGATGCGGCGATGGAAAAGCTGGGGCGTT
AA