Detailed information of TA system TA100153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1537044..1537572
• Replicon: chromosome
• Accession: NZ_CP027747
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain 66

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K10_RS06975
• Protein ID: WP_124339683.1
• Coordinates: 1537044..1537343 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7S5HLJ1
• Locus tag: C4K10_RS06980
• Protein ID: WP_038581543.1
• Coordinates: 1537333..1537572 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K10_RS06955	1532942..1533724	-	783	WP_101281929.1	aldolase	-
C4K10_RS06960	1533884..1534426	+	543	WP_007922237.1	XRE family transcriptional regulator	-
C4K10_RS06965	1534645..1535664	-	1020	WP_009047445.1	DUF4917 family protein	-
C4K10_RS06970	1535822..1536970	+	1149	WP_038630803.1	L-threonine dehydrogenase	-
C4K10_RS06975	1537044..1537343	-	300	WP_124339683.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K10_RS06980	1537333..1537572	-	240	WP_038581543.1	ribbon-helix-helix protein, CopG family	Antitoxin
C4K10_RS06985	1537709..1538125	+	417	WP_016702100.1	fosfomycin resistance glutathione transferase	-
C4K10_RS06990	1538253..1538891	+	639	WP_124339684.1	LysE family translocator	-
C4K10_RS06995	1539003..1540343	+	1341	WP_016702102.1	sensor domain-containing diguanylate cyclase	-
C4K10_RS07000	1540482..1541006	+	525	WP_124339685.1	DUF4142 domain-containing protein	-
C4K10_RS07005	1541100..1541990	-	891	WP_124339686.1	phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11514.16 Da
• Isoelectric Point: 10.8916

>T100153 WP_124339683.1 NZ_CP027747:c1537343-1537044 [Pseudomonas chlororaphis subsp. aureofaciens]
MHVEWLRNALKNLDDEAAYIAQDSPQAAAEFVKAILSSIQHLSRFPASGREGRLPGTREWVVPNRPYLIPYRVRQGRLQI
LRIFHTRRLPPAGWQDDRQ

Nucleotide

• Length: 300 bp

>T100153 NZ_CP027747:c1537343-1537044 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGCACGTTGAGTGGCTGCGCAACGCCCTCAAGAACCTCGATGACGAAGCCGCCTACATTGCACAGGACAGTCCTCAGGC
CGCCGCGGAATTCGTCAAAGCCATCCTGAGTAGCATTCAACACCTGAGCCGTTTTCCTGCCAGTGGTCGCGAAGGTCGCC
TGCCCGGTACCCGGGAGTGGGTGGTCCCCAATCGTCCTTACCTGATCCCCTACCGCGTACGTCAGGGACGCCTGCAGATA
CTGCGGATTTTCCACACCCGGCGCTTGCCGCCTGCCGGTTGGCAAGACGACCGCCAATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8648.73 Da
• Isoelectric Point: 4.5535

>AT100153 WP_038581543.1 NZ_CP027747:c1537572-1537333 [Pseudomonas chlororaphis subsp. aureofaciens]
MSVMSLRLPDDVADTLAHLAKATGRSKSFLAVDALREYLAREAWQINEIQKALTEAEAGDFASAEELDGVLNKWTGNAR

Nucleotide

• Length: 240 bp

>AT100153 NZ_CP027747:c1537572-1537333 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGTCCGTCATGTCCTTGCGCCTGCCCGATGATGTTGCCGATACCCTGGCTCACTTGGCCAAGGCGACAGGCCGCAGCAA
GTCATTCCTCGCTGTGGATGCACTGAGAGAATATCTGGCCCGCGAAGCCTGGCAAATCAACGAGATCCAGAAAGCCCTGA
CTGAAGCAGAGGCAGGCGACTTCGCCAGCGCCGAAGAGCTGGATGGCGTACTCAACAAATGGACTGGCAATGCACGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7S5HLJ1