Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 331137..331750 Replicon chromosome
Accession NZ_CP027746
Organism Pseudomonas chlororaphis subsp. aurantiaca strain DSM 19603

Toxin (Protein)


Gene name hicA Uniprot ID A0A285EYZ4
Locus tag C4K17_RS01440 Protein ID WP_009041685.1
Coordinates 331137..331319 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PT90
Locus tag C4K17_RS01445 Protein ID WP_009046450.1
Coordinates 331349..331750 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K17_RS01410 326903..327160 - 258 WP_009046444.1 hypothetical protein -
C4K17_RS01415 327213..327701 - 489 WP_028683850.1 DoxX family protein -
C4K17_RS01420 327775..328671 - 897 WP_081362987.1 SDR family oxidoreductase -
C4K17_RS01425 328700..329209 - 510 WP_081362986.1 DUF1097 domain-containing protein -
C4K17_RS01430 329243..329983 - 741 WP_081362985.1 SDR family oxidoreductase -
C4K17_RS01435 330122..331012 + 891 WP_081362984.1 LysR family transcriptional regulator -
C4K17_RS01440 331137..331319 + 183 WP_009041685.1 type II toxin-antitoxin system HicA family toxin Toxin
C4K17_RS01445 331349..331750 + 402 WP_009046450.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4K17_RS01450 331850..332896 - 1047 WP_081362983.1 LacI family DNA-binding transcriptional regulator -
C4K17_RS01455 333104..335362 + 2259 WP_081362982.1 TonB-dependent receptor -
C4K17_RS01460 335409..336734 + 1326 WP_081362981.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.08 Da        Isoelectric Point: 10.4588

>T100144 WP_009041685.1 NZ_CP027746:331137-331319 [Pseudomonas chlororaphis subsp. aurantiaca]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Download         Length: 183 bp

>T100144 NZ_CP027746:331137-331319 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCGCAGTCGGGAGCTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCAGTCAAAGGCAGTCATCACCAGTA
CAAACATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14596.44 Da        Isoelectric Point: 4.9133

>AT100144 WP_009046450.1 NZ_CP027746:331349-331750 [Pseudomonas chlororaphis subsp. aurantiaca]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT100144 NZ_CP027746:331349-331750 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCACTACGAAGGCCTGGTCGCCGATG
GTGAGCCGTTGCCCCAGGTGCATGAAGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGGGTGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCTGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGTTTTCTCGCGGCAGCGGCATTGCGCGAGTTGTCGGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285EYZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A554PT90

References