Detailed information of TA system TA100142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4143096..4143615
• Replicon: chromosome
• Accession: NZ_CP027744
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain M71

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K19_RS18620
• Protein ID: WP_124311556.1
• Coordinates: 4143096..4143377 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1H3UB71
• Locus tag: C4K19_RS18625
• Protein ID: WP_009044432.1
• Coordinates: 4143367..4143615 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K19_RS18595	4138677..4138949	+	273	WP_124311553.1	hypothetical protein	-
C4K19_RS18605	4139618..4140409	+	792	WP_038633459.1	methyltransferase domain-containing protein	-
C4K19_RS18610	4140724..4141206	+	483	WP_009049637.1	acyl-CoA thioesterase	-
C4K19_RS18615	4141263..4142912	+	1650	WP_124311555.1	FMN-binding glutamate synthase family protein	-
C4K19_RS18620	4143096..4143377	-	282	WP_124311556.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K19_RS18625	4143367..4143615	-	249	WP_009044432.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4K19_RS18630	4143712..4144500	-	789	WP_124311557.1	helix-turn-helix transcriptional regulator	-
C4K19_RS18635	4144585..4145583	+	999	WP_124311558.1	bile acid:sodium symporter	-
C4K19_RS18640	4145797..4147560	+	1764	WP_124311559.1	hypothetical protein	-
C4K19_RS18645	4147633..4148094	+	462	WP_009049642.1	YccF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10862.68 Da
• Isoelectric Point: 10.6993

>T100142 WP_124311556.1 NZ_CP027744:c4143377-4143096 [Pseudomonas chlororaphis subsp. aurantiaca]
MTYELEFSEKAWKEWQKLGPTLKEQFKKKLQERLVNPHVPADRLSGLGNAYKIKLRTAGYRLVYRVVDEVLVVTVIAVGK
RERGSAYQQARKR

Nucleotide

• Length: 282 bp

>T100142 NZ_CP027744:c4143377-4143096 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACTTATGAGCTGGAGTTCTCCGAGAAAGCCTGGAAGGAATGGCAAAAGCTGGGCCCCACGCTGAAAGAGCAATTCAA
GAAGAAGCTGCAGGAACGACTCGTCAACCCCCATGTCCCGGCCGATCGCCTGAGCGGTTTGGGCAATGCCTACAAAATCA
AACTGCGCACAGCGGGCTACCGCCTGGTGTATCGGGTGGTGGATGAGGTGTTGGTGGTGACGGTTATTGCCGTGGGCAAG
CGAGAGCGGGGGAGTGCCTATCAGCAAGCCAGGAAGCGTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8997.37 Da
• Isoelectric Point: 4.4142

>AT100142 WP_009044432.1 NZ_CP027744:c4143615-4143367 [Pseudomonas chlororaphis subsp. aurantiaca]
MTHVLLSNVVASISELKKNPMGTVAAGGGSSVAILNRNEPAFYCVPAKEYEAMMERLEDMELLALCRERENDPTIKVSLD
DL

Nucleotide

• Length: 249 bp

>AT100142 NZ_CP027744:c4143615-4143367 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACCCATGTCCTTCTGTCCAACGTCGTCGCGAGCATTTCCGAGCTCAAGAAAAACCCCATGGGCACCGTTGCGGCCGG
TGGCGGTTCCTCCGTGGCCATCCTCAATCGCAACGAACCGGCGTTTTACTGCGTTCCGGCCAAGGAATACGAAGCCATGA
TGGAGCGTCTGGAAGATATGGAGCTGCTCGCCTTGTGCCGTGAACGAGAGAATGATCCGACCATCAAGGTTTCGCTCGAT
GACTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1H3UB71