Detailed information of TA system TA100140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2347321..2347925
• Replicon: chromosome
• Accession: NZ_CP027744
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain M71

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K19_RS10915
• Protein ID: WP_164485685.1
• Coordinates: 2347758..2347925 (-)
• Length: 56 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K19_RS10910
• Protein ID: WP_124310791.1
• Coordinates: 2347321..2347722 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K19_RS10890	2343420..2345309	+	1890	WP_124310788.1	PAS domain S-box protein	-
C4K19_RS10895	2345306..2345941	+	636	WP_053260452.1	response regulator transcription factor	-
C4K19_RS10900	2346035..2346799	+	765	WP_124310789.1	hypothetical protein	-
C4K19_RS10910	2347321..2347722	-	402	WP_124310791.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K19_RS10915	2347758..2347925	-	168	WP_164485685.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K19_RS10920	2348318..2348599	-	282	WP_106696967.1	hypothetical protein	-
C4K19_RS10925	2348997..2349374	-	378	WP_124310792.1	hypothetical protein	-
C4K19_RS10930	2349664..2350038	-	375	Protein_2130	transposase	-
C4K19_RS10935	2350126..2352429	-	2304	WP_124310793.1	SIR2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2349664..2349888	224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (1-54)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 56 a.a.
• Molecular weight: 6192.28 Da
• Isoelectric Point: 10.4656

>T100140 WP_164485685.1 NZ_CP027744:c2347925-2347758 [Pseudomonas chlororaphis subsp. aurantiaca]
MIKELEAAGWVLERVTGSHHLFTHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 168 bp

>T100140 NZ_CP027744:c2347925-2347758 [Pseudomonas chlororaphis subsp. aurantiaca]
TTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTTCACGCATCCCTACCG
GCCCGAGACCGTTCCGGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGAGCCATCCAGAAACTGGCCGGGC
TGATATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14574.52 Da
• Isoelectric Point: 4.7368

>AT100140 WP_124310791.1 NZ_CP027744:c2347722-2347321 [Pseudomonas chlororaphis subsp. aurantiaca]
VHYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELARDGQAIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT100140 NZ_CP027744:c2347722-2347321 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCACTATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGATATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGTG
ACGGGCAAGCCATTCCCCTGCCCTCGCCCACCGGCACACACCGCGCCAACCCCGACTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAGACCGAGAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTGCGGGAGCACAACATCAAGAGCCGATCGTCTTTCCTGGCAGATGCGGCGATGGAAAAGCTGGGGCGTT
AA