Detailed information of TA system TA100139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1604593..1605215
• Replicon: chromosome
• Accession: NZ_CP027744
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain M71

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2N8AR08
• Locus tag: C4K19_RS07435
• Protein ID: WP_007929414.1
• Coordinates: 1605033..1605215 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K19_RS07430
• Protein ID: WP_124307846.1
• Coordinates: 1604593..1605000 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K19_RS07410	1599905..1600795	+	891	WP_124309894.1	LysR family transcriptional regulator	-
C4K19_RS07415	1601030..1602808	+	1779	WP_124307843.1	GGDEF and EAL domain-containing protein	-
C4K19_RS07420	1602842..1603756	-	915	WP_124310527.1	SGNH/GDSL hydrolase family protein	-
C4K19_RS07425	1603886..1604353	-	468	WP_124307845.1	GAF domain-containing protein	-
C4K19_RS07430	1604593..1605000	-	408	WP_124307846.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K19_RS07435	1605033..1605215	-	183	WP_007929414.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K19_RS07445	1605930..1606814	-	885	WP_124307847.1	alpha/beta hydrolase	-
C4K19_RS07450	1607166..1607858	-	693	WP_096373287.1	16S rRNA pseudouridine(516) synthase	-
C4K19_RS07455	1607892..1608125	-	234	WP_124310528.1	cysteine-rich CWC family protein	-
C4K19_RS07460	1608118..1609605	-	1488	WP_124310529.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6850.15 Da
• Isoelectric Point: 11.6709

>T100139 WP_007929414.1 NZ_CP027744:c1605215-1605033 [Pseudomonas chlororaphis subsp. aurantiaca]
VNSRYLIGQIVADGWYLVRVRGSHHHFRHPSKPGLVTVPHPKKDLLRKTAISILKQALLM

Nucleotide

• Length: 183 bp

>T100139 NZ_CP027744:c1605215-1605033 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGAACAGTCGGTATTTGATCGGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGAGGCAGTCACCACCATTT
CAGGCACCCCAGTAAACCGGGGCTGGTGACAGTTCCACATCCCAAAAAGGACCTGCTGAGAAAAACCGCCATCAGCATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14634.62 Da
• Isoelectric Point: 4.3638

>AT100139 WP_124307846.1 NZ_CP027744:c1605000-1604593 [Pseudomonas chlororaphis subsp. aurantiaca]
MLYPIAISMGDDEHAWGVEVPDIPGCFSAGDDLDDAMAMTREAIEGHFEILAEDGAAIPPANKVTLHAANPQYAGCTWAL
IDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEAKSRSGFLASAALKVLQQD

Nucleotide

• Length: 408 bp

>AT100139 NZ_CP027744:c1605000-1604593 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCTTTACCCGATTGCAATTTCAATGGGTGATGACGAACACGCCTGGGGCGTGGAAGTCCCCGACATTCCTGGCTGTTT
CTCGGCCGGCGACGACCTCGACGACGCCATGGCCATGACCCGCGAAGCCATCGAAGGGCACTTCGAGATTCTCGCCGAAG
ACGGTGCCGCCATCCCGCCCGCCAACAAGGTCACCCTGCACGCGGCCAACCCTCAGTACGCAGGCTGCACCTGGGCGCTG
ATCGACATCGATGTCACCAAGTACCTGGGCAAGGCGCAGAAGCTCAACATCACCCTGCCCGGCTACCTGCTGAACCGCAT
CGACGAATACGTGCTGCATCACCCGGAAGCGAAGAGCCGCTCGGGCTTTCTCGCTTCGGCTGCATTGAAGGTATTGCAGC
AGGACTGA

Structures

Toxin

• AlphaFold DB: A0A2N8AR08