Detailed information of TA system TA100137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 423143..423823
• Replicon: chromosome
• Accession: NZ_CP027744
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain M71

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A285GR45
• Locus tag: C4K19_RS01925
• Protein ID: WP_028681531.1
• Coordinates: 423641..423823 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K19_RS01920
• Protein ID: WP_038582934.1
• Coordinates: 423143..423568 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K19_RS01895	418865..419206	+	342	WP_124310142.1	hypothetical protein	-
C4K19_RS01900	419214..419771	+	558	WP_124310143.1	hypothetical protein	-
C4K19_RS01905	420972..421175	+	204	WP_124310144.1	hypothetical protein	-
C4K19_RS01910	421250..421879	-	630	WP_124310145.1	hypothetical protein	-
C4K19_RS01915	422345..422593	-	249	WP_124310146.1	hypothetical protein	-
C4K19_RS01920	423143..423568	-	426	WP_038582934.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K19_RS01925	423641..423823	-	183	WP_028681531.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K19_RS01930	424049..424690	+	642	WP_124310147.1	hypothetical protein	-
C4K19_RS01935	424687..425658	-	972	WP_028681533.1	hypothetical protein	-
C4K19_RS01940	425631..426335	-	705	WP_124310148.1	hypothetical protein	-
C4K19_RS01945	426332..426880	-	549	WP_124310149.1	DNA-binding protein	-
C4K19_RS01950	426877..427800	-	924	WP_124310150.1	hypothetical protein	-
C4K19_RS01955	427797..428504	-	708	WP_028681537.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	422169..452979	30810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6764.83 Da
• Isoelectric Point: 10.7304

>T100137 WP_028681531.1 NZ_CP027744:c423823-423641 [Pseudomonas chlororaphis subsp. aurantiaca]
MKYSEFRRWLKAQGAEFQPGKGSHFKVTLNGKSTVFPDHGAKEMGEGLRKSIIKQLGLKD

Nucleotide

• Length: 183 bp

>T100137 NZ_CP027744:c423823-423641 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGAAGTACAGCGAGTTTCGGCGATGGTTGAAAGCCCAAGGCGCGGAGTTCCAACCCGGCAAGGGCAGTCACTTCAAGGT
CACCTTGAATGGCAAGTCAACCGTGTTTCCAGACCACGGAGCCAAGGAAATGGGCGAAGGGTTAAGGAAGTCGATAATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15405.57 Da
• Isoelectric Point: 4.7124

>AT100137 WP_038582934.1 NZ_CP027744:c423568-423143 [Pseudomonas chlororaphis subsp. aurantiaca]
MFEYALEVHDEPGSVWLSCAEIPEMHAAGDTLGEALDGALDSIETALSIYVDERRAIPAGEPGAKAGHIVLRLPALTAAK
IALWNTLLESGMSKAELARRLDVQRPQVDRLVDFLHHSKIENVERALQELGRRISITVEAA

Nucleotide

• Length: 426 bp

>AT100137 NZ_CP027744:c423568-423143 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGTTTGAGTATGCGTTAGAGGTTCACGATGAACCGGGCAGCGTTTGGCTGTCATGTGCAGAAATCCCAGAGATGCACGC
CGCCGGCGACACTCTCGGGGAAGCCCTGGACGGTGCCCTTGATTCGATTGAGACGGCATTGTCCATCTACGTCGATGAGC
GCCGGGCCATTCCCGCCGGTGAACCAGGTGCGAAAGCGGGCCATATCGTTCTCCGTCTGCCTGCACTGACTGCGGCGAAG
ATTGCGCTGTGGAATACGCTCCTGGAGTCTGGAATGAGCAAAGCCGAGTTGGCACGCCGCCTGGACGTACAGCGGCCCCA
AGTGGATCGCTTGGTCGATTTTCTACACCACTCCAAGATTGAGAACGTCGAGCGCGCCCTGCAGGAACTCGGTCGGCGGA
TCTCTATCACCGTCGAAGCTGCATAA

Structures

Toxin

• AlphaFold DB: A0A285GR45