Detailed information of TA system TA100136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 327944..328557
• Replicon: chromosome
• Accession: NZ_CP027744
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain M71

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K19_RS01440
• Protein ID: WP_096372784.1
• Coordinates: 327944..328126 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A554PT90
• Locus tag: C4K19_RS01445
• Protein ID: WP_009046450.1
• Coordinates: 328156..328557 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K19_RS01410	323710..323967	-	258	WP_124307420.1	hypothetical protein	-
C4K19_RS01415	324020..324508	-	489	WP_124310086.1	DoxX family protein	-
C4K19_RS01420	324582..325478	-	897	WP_124310087.1	SDR family oxidoreductase	-
C4K19_RS01425	325507..326016	-	510	WP_124298260.1	DUF1097 domain-containing protein	-
C4K19_RS01430	326050..326790	-	741	WP_124307423.1	SDR family oxidoreductase	-
C4K19_RS01435	326929..327819	+	891	WP_124298261.1	LysR family transcriptional regulator	-
C4K19_RS01440	327944..328126	+	183	WP_096372784.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K19_RS01445	328156..328557	+	402	WP_009046450.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K19_RS01450	328657..329703	-	1047	WP_124310088.1	LacI family DNA-binding transcriptional regulator	-
C4K19_RS01455	329911..332169	+	2259	WP_124310089.1	TonB-dependent receptor	-
C4K19_RS01460	332216..333541	+	1326	WP_124310090.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6902.07 Da
• Isoelectric Point: 10.4588

>T100136 WP_096372784.1 NZ_CP027744:327944-328126 [Pseudomonas chlororaphis subsp. aurantiaca]
MRSRELIRMIEEDGWYLIAVKGSHHPYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Nucleotide

• Length: 183 bp

>T100136 NZ_CP027744:327944-328126 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCCGTA
CAAACATCTACACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14596.44 Da
• Isoelectric Point: 4.9133

>AT100136 WP_009046450.1 NZ_CP027744:328156-328557 [Pseudomonas chlororaphis subsp. aurantiaca]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT100136 NZ_CP027744:328156-328557 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCTGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAGCCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGCGTGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGTTTTCTCGCGGCAGCGGCATTGCGCGAGTTGTCGGCGT
AG

Structures

Antitoxin

• AlphaFold DB: A0A554PT90