Detailed information of TA system TA100135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 216910..217426
• Replicon: chromosome
• Accession: NZ_CP027744
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain M71

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: C4K19_RS00920
• Protein ID: WP_124310051.1
• Coordinates: 217145..217426 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4K19_RS00915
• Protein ID: WP_023969916.1
• Coordinates: 216910..217155 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K19_RS00890	212678..213673	+	996	WP_124310049.1	sulfotransferase family protein	-
C4K19_RS00895	213639..214397	-	759	WP_038583164.1	slipin family protein	-
C4K19_RS00900	214399..215784	-	1386	WP_124310050.1	nodulation protein NfeD	-
C4K19_RS00905	216008..216469	+	462	WP_016705004.1	YbaK/EbsC family protein	-
C4K19_RS00910	216600..216845	+	246	WP_023969915.1	DUF2789 domain-containing protein	-
C4K19_RS00915	216910..217155	+	246	WP_023969916.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
C4K19_RS00920	217145..217426	+	282	WP_124310051.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K19_RS00925	217559..217987	+	429	WP_081363042.1	winged helix DNA-binding protein	-
C4K19_RS00930	217984..220068	+	2085	WP_124310052.1	FUSC family protein	-
C4K19_RS00935	220065..220274	+	210	WP_007932080.1	DUF1656 domain-containing protein	-
C4K19_RS00940	220271..221164	+	894	WP_124310053.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10933.85 Da
• Isoelectric Point: 10.5330

>T100135 WP_124310051.1 NZ_CP027744:217145-217426 [Pseudomonas chlororaphis subsp. aurantiaca]
MTYKLQFLPSARKEWDKLGYTLREQFKRKLAERLEMPRVPADALHGMADCYKIKLKASGYRLVYQVIEDQVVVSVVAVGK
RERSAVYERARKR

Nucleotide

• Length: 282 bp

>T100135 NZ_CP027744:217145-217426 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACTTATAAGCTCCAGTTCCTGCCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTTATACCCTGCGCGAGCAATTCAA
GAGGAAGCTGGCCGAGCGCCTGGAAATGCCACGGGTGCCCGCGGACGCCCTTCACGGTATGGCGGACTGCTACAAGATCA
AGCTCAAGGCCTCTGGCTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTCGTGGTTTCGGTGGTGGCCGTGGGCAAA
CGTGAACGCAGTGCCGTGTATGAGCGGGCACGAAAGCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8840.18 Da
• Isoelectric Point: 4.7164

>AT100135 WP_023969916.1 NZ_CP027744:216910-217155 [Pseudomonas chlororaphis subsp. aurantiaca]
MQSVLADKAVSVSELKKNPSAVLNGAQGGPVAVLNHNRVMGYMVPADVFEAMMERLDDLELAEIVRARRHETPVPVNLDD
L

Nucleotide

• Length: 246 bp

>AT100135 NZ_CP027744:216910-217155 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAATCCTTCGGCTGTCCTGAACGGCGCACA
GGGTGGTCCGGTTGCGGTGCTCAATCACAACCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCCATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGTCACGAGACCCCGGTCCCGGTAAATCTGGATGAC
TTATAA