Detailed information of TA system TA100134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4183265..4183784
• Replicon: chromosome
• Accession: NZ_CP027743
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain CW2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K20_RS18565
• Protein ID: WP_124299847.1
• Coordinates: 4183265..4183546 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1H3UB71
• Locus tag: C4K20_RS18570
• Protein ID: WP_009044432.1
• Coordinates: 4183536..4183784 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K20_RS18540	4178835..4179107	+	273	WP_038578030.1	hypothetical protein	-
C4K20_RS18550	4179764..4180555	+	792	WP_124299845.1	methyltransferase domain-containing protein	-
C4K20_RS18555	4180872..4181354	+	483	WP_009049637.1	acyl-CoA thioesterase	-
C4K20_RS18560	4181411..4183060	+	1650	WP_124299846.1	FMN-binding glutamate synthase family protein	-
C4K20_RS18565	4183265..4183546	-	282	WP_124299847.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K20_RS18570	4183536..4183784	-	249	WP_009044432.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4K20_RS18575	4183880..4184668	-	789	WP_124299848.1	helix-turn-helix transcriptional regulator	-
C4K20_RS18580	4184753..4185751	+	999	WP_124299849.1	bile acid:sodium symporter	-
C4K20_RS18585	4186041..4187812	+	1772	Protein_3646	hypothetical protein	-
C4K20_RS18590	4187885..4188346	+	462	WP_009049642.1	YccF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4179764..4196313	16549

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10793.57 Da
• Isoelectric Point: 10.5816

>T100134 WP_124299847.1 NZ_CP027743:c4183546-4183265 [Pseudomonas chlororaphis subsp. aurantiaca]
MTYELEFSEKAWKEWQKLGPTLKEQFKKKLQERLVNPHVPADRLSGLGNAYKIKLRTAGYRLVYRVVDEVLVVTVIAVGK
RERGSAYQQASKR

Nucleotide

• Length: 282 bp

>T100134 NZ_CP027743:c4183546-4183265 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACTTATGAGCTGGAGTTCTCCGAGAAAGCCTGGAAGGAATGGCAAAAGCTGGGCCCCACGCTGAAAGAGCAATTCAA
GAAGAAGCTGCAGGAACGACTCGTCAACCCCCATGTCCCGGCCGATCGCCTGAGCGGTTTGGGCAATGCCTACAAAATCA
AACTGCGCACAGCGGGCTACCGCCTGGTGTATCGGGTGGTGGATGAGGTGTTGGTGGTGACGGTCATTGCCGTGGGCAAG
CGAGAGCGGGGGAGTGCCTATCAGCAAGCCAGCAAGCGTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8997.37 Da
• Isoelectric Point: 4.4142

>AT100134 WP_009044432.1 NZ_CP027743:c4183784-4183536 [Pseudomonas chlororaphis subsp. aurantiaca]
MTHVLLSNVVASISELKKNPMGTVAAGGGSSVAILNRNEPAFYCVPAKEYEAMMERLEDMELLALCRERENDPTIKVSLD
DL

Nucleotide

• Length: 249 bp

>AT100134 NZ_CP027743:c4183784-4183536 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGACCCATGTCCTTCTGTCCAACGTCGTTGCGAGCATTTCCGAGCTCAAGAAAAACCCCATGGGCACCGTTGCGGCCGG
TGGCGGTTCCTCCGTGGCCATCCTCAATCGCAACGAACCGGCGTTTTACTGCGTTCCGGCCAAGGAATACGAAGCCATGA
TGGAGCGTCTGGAAGATATGGAGCTGCTCGCCTTGTGCCGTGAACGAGAGAATGATCCGACCATCAAGGTTTCGCTCGAT
GACTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1H3UB71