Detailed information of TA system TA100133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2345636..2346255
• Replicon: chromosome
• Accession: NZ_CP027743
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain CW2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G7DMG3
• Locus tag: C4K20_RS10730
• Protein ID: WP_009048155.1
• Coordinates: 2346073..2346255 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K20_RS10725
• Protein ID: WP_103330829.1
• Coordinates: 2345636..2346037 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K20_RS10705	2341510..2343399	+	1890	WP_124298951.1	PAS domain S-box protein	-
C4K20_RS10710	2343396..2344031	+	636	WP_124298952.1	response regulator transcription factor	-
C4K20_RS10715	2344125..2344889	+	765	WP_124298953.1	hypothetical protein	-
C4K20_RS10725	2345636..2346037	-	402	WP_103330829.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K20_RS10730	2346073..2346255	-	183	WP_009048155.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K20_RS10735	2346546..2346827	-	282	WP_028682899.1	hypothetical protein	-
C4K20_RS10740	2347249..2347605	-	357	WP_103330827.1	hypothetical protein	-
C4K20_RS10745	2347590..2348372	-	783	WP_124298955.1	hypothetical protein	-
C4K20_RS10750	2348383..2348928	-	546	WP_124298956.1	PAAR domain-containing protein	-
C4K20_RS10755	2349111..2350130	-	1020	WP_124298957.1	DUF4238 domain-containing protein	-
C4K20_RS10760	2350331..2351227	-	897	WP_124298958.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2339544..2353400	13856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6799.98 Da
• Isoelectric Point: 10.9678

>T100133 WP_009048155.1 NZ_CP027743:c2346255-2346073 [Pseudomonas chlororaphis subsp. aurantiaca]
VQSRQLIKELEAAGWVLERVTGSHHLFKHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 183 bp

>T100133 NZ_CP027743:c2346255-2346073 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCAAAGCAGGCAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CAAGCATCCCTACCGGCCCGAGACCGTTCCGGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14565.51 Da
• Isoelectric Point: 4.5918

>AT100133 WP_103330829.1 NZ_CP027743:c2346037-2345636 [Pseudomonas chlororaphis subsp. aurantiaca]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELARDGQAIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT100133 NZ_CP027743:c2346037-2345636 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCTGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGTG
ACGGGCAAGCCATTCCCCTGCCCTCGCCCACCGGCACACACCGCGCCAACCCCGACTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAAACCGAGAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTGCGGGAGCACAACATCAAGAGCCGGTCATCTTTCCTGGCGGATGCGGCGATGGAAAAGCTGGGGCGTT
AA

Structures

Toxin

• AlphaFold DB: A0A3G7DMG3