Detailed information of TA system TA100132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1520857..1521385
• Replicon: chromosome
• Accession: NZ_CP027743
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain CW2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K20_RS06920
• Protein ID: WP_124300844.1
• Coordinates: 1520857..1521150 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4K20_RS06925
• Protein ID: WP_124298675.1
• Coordinates: 1521146..1521385 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K20_RS06895	1515973..1516515	+	543	WP_007922237.1	XRE family transcriptional regulator	-
C4K20_RS06900	1516842..1517861	-	1020	WP_124298674.1	DUF4917 family protein	-
C4K20_RS06905	1518019..1519167	+	1149	WP_096373264.1	L-threonine dehydrogenase	-
C4K20_RS06910	1519365..1519937	+	573	WP_081362467.1	TetR/AcrR family transcriptional regulator	-
C4K20_RS06915	1520157..1520828	+	672	WP_081362466.1	epimerase	-
C4K20_RS06920	1520857..1521150	-	294	WP_124300844.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K20_RS06925	1521146..1521385	-	240	WP_124298675.1	CopG family ribbon-helix-helix protein	Antitoxin
C4K20_RS06930	1521522..1521938	+	417	WP_124298676.1	fosfomycin resistance glutathione transferase	-
C4K20_RS06935	1522066..1522704	+	639	WP_016702101.1	LysE family translocator	-
C4K20_RS06940	1522816..1524156	+	1341	WP_124298677.1	sensor domain-containing diguanylate cyclase	-
C4K20_RS06945	1524295..1524819	+	525	WP_124298678.1	DUF4142 domain-containing protein	-
C4K20_RS06950	1524913..1525311	-	399	WP_081362462.1	low affinity iron permease family protein	-
C4K20_RS06955	1525496..1526368	-	873	WP_124298679.1	phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (1-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11222.79 Da
• Isoelectric Point: 10.1507

>T100132 WP_124300844.1 NZ_CP027743:c1521150-1520857 [Pseudomonas chlororaphis subsp. aurantiaca]
IEWLRTALKNLDEEAAYIAQDSPQAAAEFVKAILSSIQHLSHFPASGREGRLPGTREWVVPNRPYLIPYRVRQGHLQILR
IFHTRRLPPAGWQDDRQ

Nucleotide

• Length: 294 bp

>T100132 NZ_CP027743:c1521150-1520857 [Pseudomonas chlororaphis subsp. aurantiaca]
ATTGAGTGGCTGCGCACCGCCCTCAAGAACCTCGATGAAGAAGCCGCCTACATTGCACAAGACAGTCCTCAGGCCGCCGC
GGAATTCGTCAAAGCCATCCTGAGCAGCATTCAACACCTGAGCCATTTTCCTGCCAGTGGTCGCGAAGGTCGCCTGCCCG
GTACCCGGGAGTGGGTGGTACCCAACCGTCCTTACCTGATCCCCTACCGTGTACGTCAGGGACACCTGCAGATACTGCGG
ATTTTCCATACCCGGCGCTTGCCGCCTGCCGGTTGGCAAGACGACCGCCAATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8688.79 Da
• Isoelectric Point: 4.5837

>AT100132 WP_124298675.1 NZ_CP027743:c1521385-1521146 [Pseudomonas chlororaphis subsp. aurantiaca]
MSVMSLRLPDDVADTLAHLAKATGRSKSFLAVDALREYLAREAWQIDEIQKALTEAEAGDFASAEELDNVLTKWTGKAH

Nucleotide

• Length: 240 bp

>AT100132 NZ_CP027743:c1521385-1521146 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGTCCGTCATGTCCTTGCGCCTGCCCGATGATGTTGCCGATACCCTGGCTCACTTGGCCAAGGCGACAGGCCGCAGCAA
GTCATTCCTCGCTGTGGATGCACTGAGAGAATATCTGGCCCGCGAAGCCTGGCAAATCGACGAGATCCAGAAAGCCCTGA
CTGAAGCAGAGGCAGGCGACTTCGCCAGCGCCGAAGAGCTGGATAACGTGCTCACTAAATGGACTGGCAAGGCACATTGA