Detailed information of TA system TA100125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2429413..2430032
• Replicon: chromosome
• Accession: NZ_CP027742
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain 464

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K21_RS11235
• Protein ID: WP_124308109.1
• Coordinates: 2429850..2430032 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K21_RS11230
• Protein ID: WP_103330829.1
• Coordinates: 2429413..2429814 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K21_RS11210	2425271..2427160	+	1890	WP_124334436.1	PAS domain S-box protein	-
C4K21_RS11215	2427157..2427792	+	636	WP_124334437.1	response regulator transcription factor	-
C4K21_RS11220	2427886..2428650	+	765	WP_103330830.1	hypothetical protein	-
C4K21_RS11230	2429413..2429814	-	402	WP_103330829.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K21_RS11235	2429850..2430032	-	183	WP_124308109.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K21_RS11240	2430394..2430675	-	282	WP_124334438.1	hypothetical protein	-
C4K21_RS11245	2431654..2431893	+	240	WP_124334439.1	hypothetical protein	-
C4K21_RS11250	2431969..2433006	+	1038	WP_124334440.1	reverse transcriptase family protein	-
C4K21_RS11255	2433321..2433920	+	600	WP_124334441.1	hypothetical protein	-
C4K21_RS11260	2433933..2434376	+	444	WP_124334442.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2423466..2441632	18166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6787.93 Da
• Isoelectric Point: 10.9678

>T100125 WP_124308109.1 NZ_CP027742:c2430032-2429850 [Pseudomonas chlororaphis subsp. aurantiaca]
VQSRQLTKELEAAGWVLERVTGSHHLFKHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 183 bp

>T100125 NZ_CP027742:c2430032-2429850 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCAAAGCAGACAGTTGACCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CAAGCATCCCTACCGGCCGGAGACCGTTCCAGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14565.51 Da
• Isoelectric Point: 4.5918

>AT100125 WP_103330829.1 NZ_CP027742:c2429814-2429413 [Pseudomonas chlororaphis subsp. aurantiaca]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELARDGQAIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT100125 NZ_CP027742:c2429814-2429413 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGTG
ACGGGCAAGCCATTCCCCTGCCCTCACCCACCGGCACACACCGCGCCAACCCCGACTTCGAAGGGATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTGCGGGAGCACAACATCAAGAGCCGGTCGTCTTTTCTGGCGGATGCGGCGATGGAAAAGCTGGGGCGTT
AA