Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 317471..318084 Replicon chromosome
Accession NZ_CP027742
Organism Pseudomonas chlororaphis subsp. aurantiaca strain 464

Toxin (Protein)


Gene name hicA Uniprot ID A0A3G7J8U8
Locus tag C4K21_RS01410 Protein ID WP_038583007.1
Coordinates 317471..317653 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PT90
Locus tag C4K21_RS01415 Protein ID WP_009046450.1
Coordinates 317683..318084 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K21_RS01380 313237..313494 - 258 WP_038583011.1 hypothetical protein -
C4K21_RS01385 313547..314035 - 489 WP_124333842.1 DoxX family protein -
C4K21_RS01390 314109..315005 - 897 WP_124333843.1 SDR family oxidoreductase -
C4K21_RS01395 315034..315543 - 510 WP_124333844.1 DUF1097 domain-containing protein -
C4K21_RS01400 315577..316317 - 741 WP_028683849.1 SDR family oxidoreductase -
C4K21_RS01405 316456..317346 + 891 WP_081362984.1 LysR family transcriptional regulator -
C4K21_RS01410 317471..317653 + 183 WP_038583007.1 type II toxin-antitoxin system HicA family toxin Toxin
C4K21_RS01415 317683..318084 + 402 WP_009046450.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4K21_RS01420 318184..319230 - 1047 WP_081362983.1 LacI family DNA-binding transcriptional regulator -
C4K21_RS01425 319438..321696 + 2259 WP_124333845.1 TonB-dependent receptor -
C4K21_RS01430 321743..323068 + 1326 WP_124333846.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6874.01 Da        Isoelectric Point: 10.4588

>T100123 WP_038583007.1 NZ_CP027742:317471-317653 [Pseudomonas chlororaphis subsp. aurantiaca]
MRSRELIRMIEEDGWYLIAAKGSHHPYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Download         Length: 183 bp

>T100123 NZ_CP027742:317471-317653 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCGCAGTCGGGAGCTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCAGCCAAAGGCAGTCATCACCCGTA
CAAACATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14596.44 Da        Isoelectric Point: 4.9133

>AT100123 WP_009046450.1 NZ_CP027742:317683-318084 [Pseudomonas chlororaphis subsp. aurantiaca]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT100123 NZ_CP027742:317683-318084 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAAGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAGCCGCTGCCCCAGGTGCATGAAGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGGGTGATT
GAGTTCGACATCACCCCTTACTTTGGCAAGGCGGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGTTTTCTCGCGGCAGCGGCATTGCGCGAGTTGTCGGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G7J8U8

Antitoxin

Source ID Structure
AlphaFold DB A0A554PT90

References