Detailed information of TA system TA100118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2427479..2428098
• Replicon: chromosome
• Accession: NZ_CP027741
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain 449

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K22_RS11230
• Protein ID: WP_124308109.1
• Coordinates: 2427916..2428098 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K22_RS11225
• Protein ID: WP_103330829.1
• Coordinates: 2427479..2427880 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K22_RS11205	2423337..2425226	+	1890	WP_124334436.1	PAS domain S-box protein	-
C4K22_RS11210	2425223..2425858	+	636	WP_124334437.1	response regulator transcription factor	-
C4K22_RS11215	2425952..2426716	+	765	WP_103330830.1	hypothetical protein	-
C4K22_RS11225	2427479..2427880	-	402	WP_103330829.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K22_RS11230	2427916..2428098	-	183	WP_124308109.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K22_RS11235	2428460..2428741	-	282	WP_124334438.1	hypothetical protein	-
C4K22_RS11240	2429720..2429959	+	240	WP_124334439.1	hypothetical protein	-
C4K22_RS11245	2430035..2431072	+	1038	WP_124334440.1	reverse transcriptase family protein	-
C4K22_RS11250	2431387..2431986	+	600	WP_124334441.1	hypothetical protein	-
C4K22_RS11255	2431999..2432442	+	444	WP_124334442.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2421532..2439698	18166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6787.93 Da
• Isoelectric Point: 10.9678

>T100118 WP_124308109.1 NZ_CP027741:c2428098-2427916 [Pseudomonas chlororaphis subsp. aurantiaca]
VQSRQLTKELEAAGWVLERVTGSHHLFKHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 183 bp

>T100118 NZ_CP027741:c2428098-2427916 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCAAAGCAGACAGTTGACCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CAAGCATCCCTACCGGCCGGAGACCGTTCCAGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14565.51 Da
• Isoelectric Point: 4.5918

>AT100118 WP_103330829.1 NZ_CP027741:c2427880-2427479 [Pseudomonas chlororaphis subsp. aurantiaca]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELARDGQAIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT100118 NZ_CP027741:c2427880-2427479 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGTG
ACGGGCAAGCCATTCCCCTGCCCTCACCCACCGGCACACACCGCGCCAACCCCGACTTCGAAGGGATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTGCGGGAGCACAACATCAAGAGCCGGTCGTCTTTTCTGGCGGATGCGGCGATGGAAAAGCTGGGGCGTT
AA