Detailed information of TA system TA100115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 4288472..4289082
• Replicon: chromosome
• Accession: NZ_CP027740
• Organism: Pseudomonas chlororaphis subsp. piscium strain ChPhzS140

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K29_RS19225
• Protein ID: WP_124305125.1
• Coordinates: 4288780..4289082 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4K29_RS19220
• Protein ID: WP_124305124.1
• Coordinates: 4288472..4288783 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K29_RS19205	4283806..4285188	+	1383	WP_124305121.1	TIGR03752 family integrating conjugative element protein	-
C4K29_RS19210	4285169..4285579	+	411	WP_124305122.1	TIGR03751 family conjugal transfer lipoprotein	-
C4K29_RS19215	4285579..4288407	+	2829	WP_124305123.1	conjugative transfer ATPase	-
C4K29_RS19220	4288472..4288783	+	312	WP_124305124.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C4K29_RS19225	4288780..4289082	+	303	WP_124305125.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K29_RS19230	4289256..4292165	+	2910	WP_124305126.1	ATP-binding protein	-
C4K29_RS19235	4292470..4292982	+	513	WP_124305127.1	DNA repair protein RadC	-
C4K29_RS19240	4293042..4293299	+	258	WP_124305363.1	DUF1778 domain-containing protein	-
C4K29_RS19245	4293588..4293887	+	300	WP_094991432.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	spaQ / invA	4146067..4301980	155913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-54)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11696.19 Da
• Isoelectric Point: 5.1552

>T100115 WP_124305125.1 NZ_CP027740:4288780-4289082 [Pseudomonas chlororaphis subsp. piscium]
VKLEWSPWALKDRDAIFDFIEQDNPRAAIDIDSRIAEQTQRLTQFPESGRLGRCPDTRELVINRTPYIAAYRIHDGRVQI
LRVLHDAQSWPDDLPTLVER

Nucleotide

• Length: 303 bp

>T100115 NZ_CP027740:4288780-4289082 [Pseudomonas chlororaphis subsp. piscium]
GTGAAGCTGGAGTGGTCACCCTGGGCCCTGAAGGATCGCGATGCGATTTTCGACTTCATCGAACAGGACAACCCGCGTGC
CGCCATCGATATAGATAGCCGAATTGCTGAGCAGACCCAACGACTGACGCAATTCCCCGAGAGCGGTCGCCTGGGCCGTT
GCCCAGACACGCGAGAGTTGGTGATCAATCGAACACCTTACATCGCCGCCTATAGGATCCATGATGGGCGTGTGCAGATC
TTGCGCGTATTGCATGACGCGCAGAGTTGGCCGGATGATTTACCGACGTTGGTGGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11184.65 Da
• Isoelectric Point: 6.4686

>AT100115 WP_124305124.1 NZ_CP027740:4288472-4288783 [Pseudomonas chlororaphis subsp. piscium]
MASNALIQTRIDPDVKDRATAVLHNLGLTVSDAVRILLTRVANEGALPFGFATDPATYDAWFRAKVQEALDDPRPAIAAE
DVEAHFAKRRAKAQRKLSDGGEV

Nucleotide

• Length: 312 bp

>AT100115 NZ_CP027740:4288472-4288783 [Pseudomonas chlororaphis subsp. piscium]
ATGGCCAGCAACGCCCTGATCCAGACCCGCATCGACCCGGACGTGAAAGACCGCGCCACGGCAGTCCTGCACAACCTGGG
GCTGACGGTATCCGACGCCGTGCGCATCCTGCTCACCCGCGTCGCCAACGAAGGCGCCCTACCCTTCGGATTTGCCACCG
ACCCCGCCACCTATGACGCCTGGTTCCGAGCCAAAGTGCAGGAAGCCCTGGACGACCCACGCCCGGCGATTGCCGCCGAA
GATGTCGAGGCTCACTTCGCCAAGCGCCGCGCCAAGGCCCAGCGCAAGCTGTCGGATGGTGGTGAGGTGTGA